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Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil

BACKGROUND: We aimed to assess susceptibility pattern of Candida species isolated from horticulturists with onychomycosis to four antifungal drugs and to compare the effectiveness of conventional identification methods with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS)...

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Autores principales: MOBIN, Mitra, SZESZS, Maria Walderez, TAKAHASHI, Juliana Possatto, MARTINS, Marilena, de HIPPÓLITO, Daise Damaris Carnietto, PORTO, Jhonatas Cley Santos, TELES, João Batista, de LIMA, Sidney Gonçalo, MELHEM, Marcia de Souza Carvalho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379607/
https://www.ncbi.nlm.nih.gov/pubmed/30788295
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author MOBIN, Mitra
SZESZS, Maria Walderez
TAKAHASHI, Juliana Possatto
MARTINS, Marilena
de HIPPÓLITO, Daise Damaris Carnietto
PORTO, Jhonatas Cley Santos
TELES, João Batista
de LIMA, Sidney Gonçalo
MELHEM, Marcia de Souza Carvalho
author_facet MOBIN, Mitra
SZESZS, Maria Walderez
TAKAHASHI, Juliana Possatto
MARTINS, Marilena
de HIPPÓLITO, Daise Damaris Carnietto
PORTO, Jhonatas Cley Santos
TELES, João Batista
de LIMA, Sidney Gonçalo
MELHEM, Marcia de Souza Carvalho
author_sort MOBIN, Mitra
collection PubMed
description BACKGROUND: We aimed to assess susceptibility pattern of Candida species isolated from horticulturists with onychomycosis to four antifungal drugs and to compare the effectiveness of conventional identification methods with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). METHODS: This study was conducted in a community garden located in Teresina, State of Piauí, Brazil, in the year 2014. The samples were identified through phenotypic methods and per MALDI-TOF MS, being used PCR as definitive identification test. The susceptibility pattern to four antifungal drugs was determined according to Clinical and Laboratory Standards Institute (CLSI). RESULTS: Fourteen clinical isolates from seven different species were identified by the phenotypic method and by MALDI-TOF MS, with an observed concordance of 71.4% between the two methods. C. albicans (28.6%), C. parapsilosis (21.4%), C. guilliermondii and C. metapsilosis (both with 14.3%) were the most frequent species. With the exception of C. krusei, all species were sensitive to the tested antifungal. CONCLUSION: This is the first study of antifungal susceptibility of Candida in Piauí, Brazil. With the exception of C. krusei, no species showed resistance to the antifungal drugs used. This study suggests constants updates from the public databases used in MALDI-TOF MS to provide a rapid and accurate mycological diagnosis.
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spelling pubmed-63796072019-02-20 Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil MOBIN, Mitra SZESZS, Maria Walderez TAKAHASHI, Juliana Possatto MARTINS, Marilena de HIPPÓLITO, Daise Damaris Carnietto PORTO, Jhonatas Cley Santos TELES, João Batista de LIMA, Sidney Gonçalo MELHEM, Marcia de Souza Carvalho Iran J Public Health Original Article BACKGROUND: We aimed to assess susceptibility pattern of Candida species isolated from horticulturists with onychomycosis to four antifungal drugs and to compare the effectiveness of conventional identification methods with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). METHODS: This study was conducted in a community garden located in Teresina, State of Piauí, Brazil, in the year 2014. The samples were identified through phenotypic methods and per MALDI-TOF MS, being used PCR as definitive identification test. The susceptibility pattern to four antifungal drugs was determined according to Clinical and Laboratory Standards Institute (CLSI). RESULTS: Fourteen clinical isolates from seven different species were identified by the phenotypic method and by MALDI-TOF MS, with an observed concordance of 71.4% between the two methods. C. albicans (28.6%), C. parapsilosis (21.4%), C. guilliermondii and C. metapsilosis (both with 14.3%) were the most frequent species. With the exception of C. krusei, all species were sensitive to the tested antifungal. CONCLUSION: This is the first study of antifungal susceptibility of Candida in Piauí, Brazil. With the exception of C. krusei, no species showed resistance to the antifungal drugs used. This study suggests constants updates from the public databases used in MALDI-TOF MS to provide a rapid and accurate mycological diagnosis. Tehran University of Medical Sciences 2018-12 /pmc/articles/PMC6379607/ /pubmed/30788295 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
MOBIN, Mitra
SZESZS, Maria Walderez
TAKAHASHI, Juliana Possatto
MARTINS, Marilena
de HIPPÓLITO, Daise Damaris Carnietto
PORTO, Jhonatas Cley Santos
TELES, João Batista
de LIMA, Sidney Gonçalo
MELHEM, Marcia de Souza Carvalho
Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title_full Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title_fullStr Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title_full_unstemmed Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title_short Antifungal Susceptibility of Candida Species Isolated from Horticulturists with Onychomycosis in Piauí, Brazil
title_sort antifungal susceptibility of candida species isolated from horticulturists with onychomycosis in piauí, brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379607/
https://www.ncbi.nlm.nih.gov/pubmed/30788295
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