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T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
The assembly of DNA fragments with homologous arms is becoming popular in routine cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for its 3′-5′ exonuclease activity or together with a 5′-3′ exonuclease for its DNA polymerase activity. Here, we present a ‘T5 ex...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379645/ https://www.ncbi.nlm.nih.gov/pubmed/30462336 http://dx.doi.org/10.1093/nar/gky1169 |