Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation

CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA...

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Detalles Bibliográficos
Autores principales: Shen, Chih-Che, Hsu, Mu-Nung, Chang, Chin-Wei, Lin, Mei-Wei, Hwu, Jih-Ru, Tu, Yi, Hu, Yu-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379646/
https://www.ncbi.nlm.nih.gov/pubmed/30462300
http://dx.doi.org/10.1093/nar/gky1165

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379646/
https://www.ncbi.nlm.nih.gov/pubmed/30462300
http://dx.doi.org/10.1093/nar/gky1165

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