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Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation
CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379646/ https://www.ncbi.nlm.nih.gov/pubmed/30462300 http://dx.doi.org/10.1093/nar/gky1165 |