Evidence that a catalytic glutamate and an ‘Arginine Toggle’ act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor

ASCE ATPases include ring-translocases such as cellular helicases and viral DNA packaging motors (terminases). These motors have conserved Walker A and B motifs that bind Mg(2+)-ATP and a catalytic carboxylate that activates water for hydrolysis. Here we demonstrate that Glu179 serves as the catalyt...

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Detalles Bibliográficos
Autores principales: Ortiz, David, delToro, Damian, Ordyan, Mariam, Pajak, Joshua, Sippy, Jean, Catala, Alexis, Oh, Choon-Seok, Vu, Amber, Arya, Gaurav, Feiss, Michael, Smith, Douglas E, Catalano, Carlos E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379665/
https://www.ncbi.nlm.nih.gov/pubmed/30541105
http://dx.doi.org/10.1093/nar/gky1217

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