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Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms

The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitt...

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Autores principales: Cesare, Alessandra De, Parisi, Antonio, Lucchi, Alex, Capozzi, Loredana, Bianco, Angela, Pasquali, Frederique, Manfreda, Gerardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379692/
https://www.ncbi.nlm.nih.gov/pubmed/30854341
http://dx.doi.org/10.4081/ijfs.2018.7706
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author Cesare, Alessandra De
Parisi, Antonio
Lucchi, Alex
Capozzi, Loredana
Bianco, Angela
Pasquali, Frederique
Manfreda, Gerardo
author_facet Cesare, Alessandra De
Parisi, Antonio
Lucchi, Alex
Capozzi, Loredana
Bianco, Angela
Pasquali, Frederique
Manfreda, Gerardo
author_sort Cesare, Alessandra De
collection PubMed
description The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses. Firmicutes displayed abundances always higher than 38% but did not show statistically significative differences between conventional and antibiotic free carcasses. On the contrary, Bacteroidetes and Actinobacteria were significantly higher in antibiotic free then conventional carcasses (21.57 vs 10.95%; 19.29 vs 12.05%), whereas Proteobacteria were higher in the latter (33.19 vs 19.52%). The genera significantly higher in antibiotic free than conventional carcasses were Chryseobacterium (10.07 vs 1.94%), Rothia (3.08 vs 0.77%) and Micrococcus (1.12 vs 0.16%), while Shewanella was significantly higher in conventional carcasses (1.38 vs 0.26%). Among Firmicutes, the genera significantly higher in conventional carcasses were Ureibacillus (1.45 vs 0.11%) and Bacillus (3.28 vs 0.56%). The higher abundance of Proteobacteria in conventional carcasses might suggest that hygienic conditions in conventional farms are worse than antibiotic free farms. However, from a food safety point of view, Salmonella was not detected in both kinds of carcasses and the Campylobacter mean relative frequency of abundance was always lower than 0.4%.
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spelling pubmed-63796922019-03-08 Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms Cesare, Alessandra De Parisi, Antonio Lucchi, Alex Capozzi, Loredana Bianco, Angela Pasquali, Frederique Manfreda, Gerardo Ital J Food Saf Article The aim of this study was to assess microbiota and microbiological hazards in poultry carcasses from animals reared in conventional (n=15) and antibiotic free (n=15) farms. An aliquot of neck and breast skin was obtained from each individual carcass at the end of the refrigeration tunnel and submitted to DNA extraction. Total DNA was sequenced in the 16S rRNA and reads analysed with MG-RAST to classify the colonising bacteria up to the genus level and compare each taxonomic group in terms of mean relative frequency of abundance in conventional and antibiotic free carcasses. Firmicutes displayed abundances always higher than 38% but did not show statistically significative differences between conventional and antibiotic free carcasses. On the contrary, Bacteroidetes and Actinobacteria were significantly higher in antibiotic free then conventional carcasses (21.57 vs 10.95%; 19.29 vs 12.05%), whereas Proteobacteria were higher in the latter (33.19 vs 19.52%). The genera significantly higher in antibiotic free than conventional carcasses were Chryseobacterium (10.07 vs 1.94%), Rothia (3.08 vs 0.77%) and Micrococcus (1.12 vs 0.16%), while Shewanella was significantly higher in conventional carcasses (1.38 vs 0.26%). Among Firmicutes, the genera significantly higher in conventional carcasses were Ureibacillus (1.45 vs 0.11%) and Bacillus (3.28 vs 0.56%). The higher abundance of Proteobacteria in conventional carcasses might suggest that hygienic conditions in conventional farms are worse than antibiotic free farms. However, from a food safety point of view, Salmonella was not detected in both kinds of carcasses and the Campylobacter mean relative frequency of abundance was always lower than 0.4%. PAGEPress Publications, Pavia, Italy 2019-02-11 /pmc/articles/PMC6379692/ /pubmed/30854341 http://dx.doi.org/10.4081/ijfs.2018.7706 Text en ©Copyright A. De Cesare et al., 2018 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Cesare, Alessandra De
Parisi, Antonio
Lucchi, Alex
Capozzi, Loredana
Bianco, Angela
Pasquali, Frederique
Manfreda, Gerardo
Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_full Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_fullStr Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_full_unstemmed Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_short Microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
title_sort microbiota analysis and microbiological hazard assessment in poultry carcasses from conventional and antibiotic free farms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379692/
https://www.ncbi.nlm.nih.gov/pubmed/30854341
http://dx.doi.org/10.4081/ijfs.2018.7706
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