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Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis
Recently, due to the dual roles of DNA and enzyme, DNAzyme has been widely used in the field of DNA circuit, which has a wide range of applications in bio-engineered system, information processing and biocomputing. In fact, the activity of DNAzymes was regulated by subunits assembly, pH control and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379719/ https://www.ncbi.nlm.nih.gov/pubmed/30541100 http://dx.doi.org/10.1093/nar/gky1245 |
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author | Zheng, Xuedong Yang, Jing Zhou, Changjun Zhang, Cheng Zhang, Qiang Wei, Xiaopeng |
author_facet | Zheng, Xuedong Yang, Jing Zhou, Changjun Zhang, Cheng Zhang, Qiang Wei, Xiaopeng |
author_sort | Zheng, Xuedong |
collection | PubMed |
description | Recently, due to the dual roles of DNA and enzyme, DNAzyme has been widely used in the field of DNA circuit, which has a wide range of applications in bio-engineered system, information processing and biocomputing. In fact, the activity of DNAzymes was regulated by subunits assembly, pH control and metal ions triggers. However, those regulations required to change the sequences of whole DNAzyme, as separating parts and inserting extra DNA sequence. Inspired by the allosteric regulation of proteins in nature, a new allosteric strategy is proposed to regulate the activity of DNAzyme without DNA sequences changes. In this strategy, DNA strand displacement was used to regulate the DNAzyme structure, through which the activity of DNAzyme was well controlled. The strategy was applied to E6-type DNAzymes, and the operations of DNA logic circuit (YES, OR, AND, cascading and feedback) were established and simulated with the dynamic analyses. The allosteric regulation has potential to construct more complicated molecular systems, which can be applied to bio-sensing and detection. |
format | Online Article Text |
id | pubmed-6379719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-63797192019-02-22 Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis Zheng, Xuedong Yang, Jing Zhou, Changjun Zhang, Cheng Zhang, Qiang Wei, Xiaopeng Nucleic Acids Res Chemical Biology and Nucleic Acid Chemistry Recently, due to the dual roles of DNA and enzyme, DNAzyme has been widely used in the field of DNA circuit, which has a wide range of applications in bio-engineered system, information processing and biocomputing. In fact, the activity of DNAzymes was regulated by subunits assembly, pH control and metal ions triggers. However, those regulations required to change the sequences of whole DNAzyme, as separating parts and inserting extra DNA sequence. Inspired by the allosteric regulation of proteins in nature, a new allosteric strategy is proposed to regulate the activity of DNAzyme without DNA sequences changes. In this strategy, DNA strand displacement was used to regulate the DNAzyme structure, through which the activity of DNAzyme was well controlled. The strategy was applied to E6-type DNAzymes, and the operations of DNA logic circuit (YES, OR, AND, cascading and feedback) were established and simulated with the dynamic analyses. The allosteric regulation has potential to construct more complicated molecular systems, which can be applied to bio-sensing and detection. Oxford University Press 2019-02-20 2018-12-12 /pmc/articles/PMC6379719/ /pubmed/30541100 http://dx.doi.org/10.1093/nar/gky1245 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Chemical Biology and Nucleic Acid Chemistry Zheng, Xuedong Yang, Jing Zhou, Changjun Zhang, Cheng Zhang, Qiang Wei, Xiaopeng Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title | Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title_full | Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title_fullStr | Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title_full_unstemmed | Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title_short | Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis |
title_sort | allosteric dnazyme-based dna logic circuit: operations and dynamic analysis |
topic | Chemical Biology and Nucleic Acid Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379719/ https://www.ncbi.nlm.nih.gov/pubmed/30541100 http://dx.doi.org/10.1093/nar/gky1245 |
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