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Evidence for the involvement of FXR signaling in ovarian granulosa cell function

Farnesoid X receptor (FXR) is mainly present in enterohepatic tissues and regulates cholesterol, lipid, and glucose homeostasis in coordination with target genes such as SHP and FABP6. Although FXR has been revealed to be expressed in reproductive tissues, FXR function and expression levels in the o...

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Autores principales: TAKAE, Kentaro, NAKATA, Mizuho, WATANABE, Takafumi, SASADA, Hiroshi, FUJII, Hiroshi, TOMIOKA, Ikuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379767/
https://www.ncbi.nlm.nih.gov/pubmed/30449821
http://dx.doi.org/10.1262/jrd.2018-054
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author TAKAE, Kentaro
NAKATA, Mizuho
WATANABE, Takafumi
SASADA, Hiroshi
FUJII, Hiroshi
TOMIOKA, Ikuo
author_facet TAKAE, Kentaro
NAKATA, Mizuho
WATANABE, Takafumi
SASADA, Hiroshi
FUJII, Hiroshi
TOMIOKA, Ikuo
author_sort TAKAE, Kentaro
collection PubMed
description Farnesoid X receptor (FXR) is mainly present in enterohepatic tissues and regulates cholesterol, lipid, and glucose homeostasis in coordination with target genes such as SHP and FABP6. Although FXR has been revealed to be expressed in reproductive tissues, FXR function and expression levels in the ovary remain unknown. In this study, we investigated FXR expression in mouse ovaries and its target genes in ovarian granulosa cells. In situ hybridization and immunohistochemical staining showed that FXR was mainly distributed in secondary and tertiary follicles. The agonist-induced activation of FXR in cultured granulosa cells induced the expression of SHP and FABP6, while siRNA targeting of FXR decreased CYP19a1 and HSD17b1 expression. Upon examination of the roles of SHP and FABP6 in granulosa cells, we found that SHP overexpression significantly decreased StAR, CYP11a1, and HSD3b gene expression. In addition, siRNA targeting of FABP6 decreased CYP19a1 and HSD17b1 expression, while FABP6 overexpression increased CYP19a1 expression. In conclusion, the present study demonstrates the presence of FXR signaling in the ovary and reveals that FXR signaling may have a role in function of granulosa cells.
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spelling pubmed-63797672019-02-22 Evidence for the involvement of FXR signaling in ovarian granulosa cell function TAKAE, Kentaro NAKATA, Mizuho WATANABE, Takafumi SASADA, Hiroshi FUJII, Hiroshi TOMIOKA, Ikuo J Reprod Dev Original Article Farnesoid X receptor (FXR) is mainly present in enterohepatic tissues and regulates cholesterol, lipid, and glucose homeostasis in coordination with target genes such as SHP and FABP6. Although FXR has been revealed to be expressed in reproductive tissues, FXR function and expression levels in the ovary remain unknown. In this study, we investigated FXR expression in mouse ovaries and its target genes in ovarian granulosa cells. In situ hybridization and immunohistochemical staining showed that FXR was mainly distributed in secondary and tertiary follicles. The agonist-induced activation of FXR in cultured granulosa cells induced the expression of SHP and FABP6, while siRNA targeting of FXR decreased CYP19a1 and HSD17b1 expression. Upon examination of the roles of SHP and FABP6 in granulosa cells, we found that SHP overexpression significantly decreased StAR, CYP11a1, and HSD3b gene expression. In addition, siRNA targeting of FABP6 decreased CYP19a1 and HSD17b1 expression, while FABP6 overexpression increased CYP19a1 expression. In conclusion, the present study demonstrates the presence of FXR signaling in the ovary and reveals that FXR signaling may have a role in function of granulosa cells. The Society for Reproduction and Development 2018-11-16 2019-02 /pmc/articles/PMC6379767/ /pubmed/30449821 http://dx.doi.org/10.1262/jrd.2018-054 Text en ©2019 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
TAKAE, Kentaro
NAKATA, Mizuho
WATANABE, Takafumi
SASADA, Hiroshi
FUJII, Hiroshi
TOMIOKA, Ikuo
Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title_full Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title_fullStr Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title_full_unstemmed Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title_short Evidence for the involvement of FXR signaling in ovarian granulosa cell function
title_sort evidence for the involvement of fxr signaling in ovarian granulosa cell function
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379767/
https://www.ncbi.nlm.nih.gov/pubmed/30449821
http://dx.doi.org/10.1262/jrd.2018-054
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