Cargando…
Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA
The present study has synthesized poly(4,4'-cyclohexylidene bisphenol oxalate) by the condensation of oxalyl chloride with 4,4'-cyclohexylidene bisphenol, where its efficacy was tested for the solid-phase extraction of DNA. The synthesized polymer in the form of a white powder was characte...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379882/ https://www.ncbi.nlm.nih.gov/pubmed/30868072 http://dx.doi.org/10.1155/2019/7064073 |
_version_ | 1783396207394029568 |
---|---|
author | Al balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh, Helmi Al Abbosh, Khulood Fahad Al-Lohedan, Hamad A. |
author_facet | Al balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh, Helmi Al Abbosh, Khulood Fahad Al-Lohedan, Hamad A. |
author_sort | Al balawi, Aisha Nawaf |
collection | PubMed |
description | The present study has synthesized poly(4,4'-cyclohexylidene bisphenol oxalate) by the condensation of oxalyl chloride with 4,4'-cyclohexylidene bisphenol, where its efficacy was tested for the solid-phase extraction of DNA. The synthesized polymer in the form of a white powder was characterized by FTIR, TGA-DTG, SEM, and BET analysis. The study utilized solid-phase application of the resulting polymer to extract DNA. The analysis of results provided the information that the extraction efficiency is a strong dependent of polymer amount and binding buffer type. Among the three types of buffers tested, the GuHCl buffer produced the most satisfactory results in terms of yield and efficiency of extraction. Moreover, the absorbance ratio of A260/A280 in all of the samples varied from 1.682 to 1.491, thereby confirming the capability of poly(4,4'-cyclohexylidene bisphenol oxalate) to elute pure DNA. The results demonstrated an increased DNA binding capacity with respect to increased percentage of the polymer. The study has concluded that poly(bisphenol Z oxalate) can be applied as one of the potential candidates for the high efficiency extraction of DNA by means of a simple, cost-effective, and environmentally friendly approach compared to the other traditional solid-phase methods. |
format | Online Article Text |
id | pubmed-6379882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63798822019-03-13 Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA Al balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh, Helmi Al Abbosh, Khulood Fahad Al-Lohedan, Hamad A. Biomed Res Int Research Article The present study has synthesized poly(4,4'-cyclohexylidene bisphenol oxalate) by the condensation of oxalyl chloride with 4,4'-cyclohexylidene bisphenol, where its efficacy was tested for the solid-phase extraction of DNA. The synthesized polymer in the form of a white powder was characterized by FTIR, TGA-DTG, SEM, and BET analysis. The study utilized solid-phase application of the resulting polymer to extract DNA. The analysis of results provided the information that the extraction efficiency is a strong dependent of polymer amount and binding buffer type. Among the three types of buffers tested, the GuHCl buffer produced the most satisfactory results in terms of yield and efficiency of extraction. Moreover, the absorbance ratio of A260/A280 in all of the samples varied from 1.682 to 1.491, thereby confirming the capability of poly(4,4'-cyclohexylidene bisphenol oxalate) to elute pure DNA. The results demonstrated an increased DNA binding capacity with respect to increased percentage of the polymer. The study has concluded that poly(bisphenol Z oxalate) can be applied as one of the potential candidates for the high efficiency extraction of DNA by means of a simple, cost-effective, and environmentally friendly approach compared to the other traditional solid-phase methods. Hindawi 2019-02-05 /pmc/articles/PMC6379882/ /pubmed/30868072 http://dx.doi.org/10.1155/2019/7064073 Text en Copyright © 2019 Aisha Nawaf Al balawi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Al balawi, Aisha Nawaf Yusof, Nor Azah Kamaruzaman, Sazlinda Mohammad, Faruq Wasoh, Helmi Al Abbosh, Khulood Fahad Al-Lohedan, Hamad A. Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title | Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title_full | Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title_fullStr | Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title_full_unstemmed | Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title_short | Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA |
title_sort | synthesis, characterization, and application of poly(4,4'-cyclohexylidene bisphenol oxalate) for solid-phase extraction of dna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379882/ https://www.ncbi.nlm.nih.gov/pubmed/30868072 http://dx.doi.org/10.1155/2019/7064073 |
work_keys_str_mv | AT albalawiaishanawaf synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT yusofnorazah synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT kamaruzamansazlinda synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT mohammadfaruq synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT wasohhelmi synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT alabboshkhuloodfahad synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna AT allohedanhamada synthesischaracterizationandapplicationofpoly44cyclohexylidenebisphenoloxalateforsolidphaseextractionofdna |