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Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss)
Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (S...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380033/ https://www.ncbi.nlm.nih.gov/pubmed/30777130 http://dx.doi.org/10.1186/s13567-019-0632-4 |
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author | Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Krudtaa Rimstad, Espen Olesen, Niels Jørgen |
author_facet | Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Krudtaa Rimstad, Espen Olesen, Niels Jørgen |
author_sort | Vendramin, Niccoló |
collection | PubMed |
description | Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-019-0632-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6380033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63800332019-02-28 Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Krudtaa Rimstad, Espen Olesen, Niels Jørgen Vet Res Research Article Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-019-0632-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-18 2019 /pmc/articles/PMC6380033/ /pubmed/30777130 http://dx.doi.org/10.1186/s13567-019-0632-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Krudtaa Rimstad, Espen Olesen, Niels Jørgen Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title | Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full | Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_fullStr | Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed | Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_short | Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_sort | piscine orthoreovirus subtype 3 (prv-3) causes heart inflammation in rainbow trout (oncorhynchus mykiss) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380033/ https://www.ncbi.nlm.nih.gov/pubmed/30777130 http://dx.doi.org/10.1186/s13567-019-0632-4 |
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