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Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis

BACKGROUND: Curcuma longa has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with C. longa extract in early pregnant mice with acute toxoplasmosis. MATERIALS AND METHODS: We evalua...

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Autores principales: Simanjuntak, Tigor Peniel, Hatta, Mochammad, Tahir, Andi M., Sirait, Robert H., Karo, Marni B., Tambaib, Titus, Dwiyanti, Ressy, Noviyanthi, Rizki Amelia, Junita, Ade Rifka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380095/
https://www.ncbi.nlm.nih.gov/pubmed/30814832
http://dx.doi.org/10.4103/jgid.jgid_28_18
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author Simanjuntak, Tigor Peniel
Hatta, Mochammad
Tahir, Andi M.
Sirait, Robert H.
Karo, Marni B.
Tambaib, Titus
Dwiyanti, Ressy
Noviyanthi, Rizki Amelia
Junita, Ade Rifka
author_facet Simanjuntak, Tigor Peniel
Hatta, Mochammad
Tahir, Andi M.
Sirait, Robert H.
Karo, Marni B.
Tambaib, Titus
Dwiyanti, Ressy
Noviyanthi, Rizki Amelia
Junita, Ade Rifka
author_sort Simanjuntak, Tigor Peniel
collection PubMed
description BACKGROUND: Curcuma longa has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with C. longa extract in early pregnant mice with acute toxoplasmosis. MATERIALS AND METHODS: We evaluated 20 early pregnant mice that were divided into five groups, four mice in each. Group 1-4 received injections of Toxoplasma gondii tachyzoites. Three days later, G1 and G2 were given orally 125 mg/kg/day and 500 mg/kg/day of C. longa extract, respectively. G3 was given 60 mg/kg/day of spiramycin (positive control), and G4 was given 0.2 ml of distilled water (negative control). G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 days and 7 days after intervention) to assess anti-Toxoplasma IgG-IgM antibody levels by enzyme-linked immunosorbent assay methods. RESULTS: Anti-Toxoplasma IgG-IgM antibody levels increased significantly 3 days after injection of tachyzoites (P < 0.05), but decreased significantly (P < 0.05) 3 days, and 7 days after administration of C. longa extract dose 125 mg, 500 mg, and spiramycin 60 mg, and there was no significant difference between these three groups. Anti-Toxoplasma IgG-IgM antibody levels increased significantly (P < 0.05) 3 days, 6 days, and 10 days after injections of tachyzoites on G4. The IgG-IgM antibody levels fluctuated on G5 and considered as insignificant (P > 0.05). CONCLUSION: The administration of C. longa extract at a dose of 125 mg/kg/day for 7 days effectively decreased anti-Toxoplasma IgG-IgM antibody level in early pregnant mice with acute toxoplasmosis.
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spelling pubmed-63800952019-02-27 Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis Simanjuntak, Tigor Peniel Hatta, Mochammad Tahir, Andi M. Sirait, Robert H. Karo, Marni B. Tambaib, Titus Dwiyanti, Ressy Noviyanthi, Rizki Amelia Junita, Ade Rifka J Glob Infect Dis Original Article BACKGROUND: Curcuma longa has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with C. longa extract in early pregnant mice with acute toxoplasmosis. MATERIALS AND METHODS: We evaluated 20 early pregnant mice that were divided into five groups, four mice in each. Group 1-4 received injections of Toxoplasma gondii tachyzoites. Three days later, G1 and G2 were given orally 125 mg/kg/day and 500 mg/kg/day of C. longa extract, respectively. G3 was given 60 mg/kg/day of spiramycin (positive control), and G4 was given 0.2 ml of distilled water (negative control). G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 days and 7 days after intervention) to assess anti-Toxoplasma IgG-IgM antibody levels by enzyme-linked immunosorbent assay methods. RESULTS: Anti-Toxoplasma IgG-IgM antibody levels increased significantly 3 days after injection of tachyzoites (P < 0.05), but decreased significantly (P < 0.05) 3 days, and 7 days after administration of C. longa extract dose 125 mg, 500 mg, and spiramycin 60 mg, and there was no significant difference between these three groups. Anti-Toxoplasma IgG-IgM antibody levels increased significantly (P < 0.05) 3 days, 6 days, and 10 days after injections of tachyzoites on G4. The IgG-IgM antibody levels fluctuated on G5 and considered as insignificant (P > 0.05). CONCLUSION: The administration of C. longa extract at a dose of 125 mg/kg/day for 7 days effectively decreased anti-Toxoplasma IgG-IgM antibody level in early pregnant mice with acute toxoplasmosis. Medknow Publications & Media Pvt Ltd 2019 /pmc/articles/PMC6380095/ /pubmed/30814832 http://dx.doi.org/10.4103/jgid.jgid_28_18 Text en Copyright: © 2019 Journal of Global Infectious Diseases http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Simanjuntak, Tigor Peniel
Hatta, Mochammad
Tahir, Andi M.
Sirait, Robert H.
Karo, Marni B.
Tambaib, Titus
Dwiyanti, Ressy
Noviyanthi, Rizki Amelia
Junita, Ade Rifka
Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title_full Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title_fullStr Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title_full_unstemmed Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title_short Analysis of Anti-toxoplasma Immunoglobulin G and Immunoglobulin M Antibody Levels after Intervention with Curcuma Longa Extract on Early Pregnant Mice with Acute Toxoplasmosis
title_sort analysis of anti-toxoplasma immunoglobulin g and immunoglobulin m antibody levels after intervention with curcuma longa extract on early pregnant mice with acute toxoplasmosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380095/
https://www.ncbi.nlm.nih.gov/pubmed/30814832
http://dx.doi.org/10.4103/jgid.jgid_28_18
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