Cargando…

Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation

The Yiqi-Huoxue granule (YQHX) is a traditional Chinese medication widely used in the therapy of the traditional Chinese medicine diagnosis “Qi deficiency” or “blood stasis” in China. Both these symptoms are related to inflammation, but the mechanisms of YQHX against inflammation are largely unknown...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Hong, Wang, Xinzhou, Gao, Shuibo, Dai, Liping, Tong, Haibin, Gao, Haixia, Lei, Zhen, Han, Yongjun, Wang, Zhentao, Han, Lihua, Qi, Dake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381561/
https://www.ncbi.nlm.nih.gov/pubmed/30881601
http://dx.doi.org/10.1155/2019/9425183
_version_ 1783396522823516160
author Wu, Hong
Wang, Xinzhou
Gao, Shuibo
Dai, Liping
Tong, Haibin
Gao, Haixia
Lei, Zhen
Han, Yongjun
Wang, Zhentao
Han, Lihua
Qi, Dake
author_facet Wu, Hong
Wang, Xinzhou
Gao, Shuibo
Dai, Liping
Tong, Haibin
Gao, Haixia
Lei, Zhen
Han, Yongjun
Wang, Zhentao
Han, Lihua
Qi, Dake
author_sort Wu, Hong
collection PubMed
description The Yiqi-Huoxue granule (YQHX) is a traditional Chinese medication widely used in the therapy of the traditional Chinese medicine diagnosis “Qi deficiency” or “blood stasis” in China. Both these symptoms are related to inflammation, but the mechanisms of YQHX against inflammation are largely unknown. Thus, our present study investigated the effects of YQHX on regulating inflammatory responses induced by lipopolysaccharides (LPS) in HUVECs. Our data found that YQHX remarkably inhibits the production of prothrombotic factors, plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF), while it upregulates the protein expression of Kruppel-like factor 2 (KLF2). The increase in PAI-1 and TF was significantly attenuated through a transgenic knockdown in KLF2 with a Lenti-shKLF2 vector. YQHX also decreases the phosphorylation of nuclear factor-κB (NF-κB) p65 and IκB following LPS stimulation, and it effectively suppresses PAI-1 and TF via a NF-κB-dependent mechanism. Taken together, our results suggest that YQHX provides a notable antithrombotic activity via regulating the KLF2 expression and NF-κB signaling pathway in HUVECs. The KLF2 and NF-κB may be potential therapeutic targets for interventions of inflammation associated with atherosclerosis.
format Online
Article
Text
id pubmed-6381561
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-63815612019-03-17 Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation Wu, Hong Wang, Xinzhou Gao, Shuibo Dai, Liping Tong, Haibin Gao, Haixia Lei, Zhen Han, Yongjun Wang, Zhentao Han, Lihua Qi, Dake Oxid Med Cell Longev Research Article The Yiqi-Huoxue granule (YQHX) is a traditional Chinese medication widely used in the therapy of the traditional Chinese medicine diagnosis “Qi deficiency” or “blood stasis” in China. Both these symptoms are related to inflammation, but the mechanisms of YQHX against inflammation are largely unknown. Thus, our present study investigated the effects of YQHX on regulating inflammatory responses induced by lipopolysaccharides (LPS) in HUVECs. Our data found that YQHX remarkably inhibits the production of prothrombotic factors, plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF), while it upregulates the protein expression of Kruppel-like factor 2 (KLF2). The increase in PAI-1 and TF was significantly attenuated through a transgenic knockdown in KLF2 with a Lenti-shKLF2 vector. YQHX also decreases the phosphorylation of nuclear factor-κB (NF-κB) p65 and IκB following LPS stimulation, and it effectively suppresses PAI-1 and TF via a NF-κB-dependent mechanism. Taken together, our results suggest that YQHX provides a notable antithrombotic activity via regulating the KLF2 expression and NF-κB signaling pathway in HUVECs. The KLF2 and NF-κB may be potential therapeutic targets for interventions of inflammation associated with atherosclerosis. Hindawi 2019-02-06 /pmc/articles/PMC6381561/ /pubmed/30881601 http://dx.doi.org/10.1155/2019/9425183 Text en Copyright © 2019 Hong Wu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wu, Hong
Wang, Xinzhou
Gao, Shuibo
Dai, Liping
Tong, Haibin
Gao, Haixia
Lei, Zhen
Han, Yongjun
Wang, Zhentao
Han, Lihua
Qi, Dake
Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title_full Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title_fullStr Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title_full_unstemmed Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title_short Yiqi-Huoxue Granule (YQHX) Downregulates Prothrombotic Factors by Modulating KLF2 and NF-κB in HUVECs following LPS Stimulation
title_sort yiqi-huoxue granule (yqhx) downregulates prothrombotic factors by modulating klf2 and nf-κb in huvecs following lps stimulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381561/
https://www.ncbi.nlm.nih.gov/pubmed/30881601
http://dx.doi.org/10.1155/2019/9425183
work_keys_str_mv AT wuhong yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT wangxinzhou yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT gaoshuibo yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT dailiping yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT tonghaibin yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT gaohaixia yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT leizhen yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT hanyongjun yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT wangzhentao yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT hanlihua yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation
AT qidake yiqihuoxuegranuleyqhxdownregulatesprothromboticfactorsbymodulatingklf2andnfkbinhuvecsfollowinglpsstimulation