Cargando…

Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy

The concentration of thiobarbituric acid reactive substances (TBARSs) in plasma and erythrocytes, the activity of selected antioxidant enzymes in erythrocytes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and the levels of hemoglobin (HGB) and haematocrit (HCT) were...

Descripción completa

Detalles Bibliográficos
Autores principales: Paprocki, Jarosław, Sutkowy, Paweł, Piechocki, Jacek, Woźniak, Alina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381569/
https://www.ncbi.nlm.nih.gov/pubmed/30881599
http://dx.doi.org/10.1155/2019/8472346
_version_ 1783396525005602816
author Paprocki, Jarosław
Sutkowy, Paweł
Piechocki, Jacek
Woźniak, Alina
author_facet Paprocki, Jarosław
Sutkowy, Paweł
Piechocki, Jacek
Woźniak, Alina
author_sort Paprocki, Jarosław
collection PubMed
description The concentration of thiobarbituric acid reactive substances (TBARSs) in plasma and erythrocytes, the activity of selected antioxidant enzymes in erythrocytes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and the levels of hemoglobin (HGB) and haematocrit (HCT) were determined in 40 patients with sudden sensorineural hearing loss (SSNHL) subjected to 14 treatment sessions in a Haux Starmed 2200 hyperbaric chamber. Hyperbaric oxygen (HBO) therapy involved breathing 100% oxygen at 0.25 MPa. Blood for analysis was collected from the basilic vein at three time points: before the first HBO session, approximately 5 min after the first session, and after the 14th session. The control group included 20 healthy individuals never before treated with HBO therapy. Compared to the pre-HBO values, a 10% increase (P < 0.05) in the TBARS concentration in erythrocytes, a 28% increase in the GPx activity (P < 0.05), and a 7% decrease in the SOD activity (P < 0.05) were observed after 14 HBO sessions. The CAT activity decreased by 6% (P < 0.05) after the first session. The TBARS concentration in plasma was 13% higher (P < 0.01), while that in erythrocytes was 24% lower (P < 0.001) in the SSNHL patients before the first HBO session compared to the control group. The CAT activity in the SSNHL patients before HBO therapy was 26% higher (P < 0.001) than that in the control group. A statistically significant reduction in HGB and HCT after 14 HBO sessions (P < 0.01) compared to the pre-HBO values was demonstrated. SSNHL is accompanied by disturbance in the oxidant-antioxidant equilibrium. Repeated stimulation with hyperbaric oxygen modulates the activity of antioxidant enzymes. It seems that the increased generation of hydrogen peroxide is responsible for the changes in the activity of antioxidant barrier enzymes observed after HBO sessions.
format Online
Article
Text
id pubmed-6381569
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-63815692019-03-17 Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy Paprocki, Jarosław Sutkowy, Paweł Piechocki, Jacek Woźniak, Alina Oxid Med Cell Longev Research Article The concentration of thiobarbituric acid reactive substances (TBARSs) in plasma and erythrocytes, the activity of selected antioxidant enzymes in erythrocytes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and the levels of hemoglobin (HGB) and haematocrit (HCT) were determined in 40 patients with sudden sensorineural hearing loss (SSNHL) subjected to 14 treatment sessions in a Haux Starmed 2200 hyperbaric chamber. Hyperbaric oxygen (HBO) therapy involved breathing 100% oxygen at 0.25 MPa. Blood for analysis was collected from the basilic vein at three time points: before the first HBO session, approximately 5 min after the first session, and after the 14th session. The control group included 20 healthy individuals never before treated with HBO therapy. Compared to the pre-HBO values, a 10% increase (P < 0.05) in the TBARS concentration in erythrocytes, a 28% increase in the GPx activity (P < 0.05), and a 7% decrease in the SOD activity (P < 0.05) were observed after 14 HBO sessions. The CAT activity decreased by 6% (P < 0.05) after the first session. The TBARS concentration in plasma was 13% higher (P < 0.01), while that in erythrocytes was 24% lower (P < 0.001) in the SSNHL patients before the first HBO session compared to the control group. The CAT activity in the SSNHL patients before HBO therapy was 26% higher (P < 0.001) than that in the control group. A statistically significant reduction in HGB and HCT after 14 HBO sessions (P < 0.01) compared to the pre-HBO values was demonstrated. SSNHL is accompanied by disturbance in the oxidant-antioxidant equilibrium. Repeated stimulation with hyperbaric oxygen modulates the activity of antioxidant enzymes. It seems that the increased generation of hydrogen peroxide is responsible for the changes in the activity of antioxidant barrier enzymes observed after HBO sessions. Hindawi 2019-02-06 /pmc/articles/PMC6381569/ /pubmed/30881599 http://dx.doi.org/10.1155/2019/8472346 Text en Copyright © 2019 Jarosław Paprocki et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Paprocki, Jarosław
Sutkowy, Paweł
Piechocki, Jacek
Woźniak, Alina
Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title_full Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title_fullStr Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title_full_unstemmed Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title_short Markers of Oxidant-Antioxidant Equilibrium in Patients with Sudden Sensorineural Hearing Loss Treated with Hyperbaric Oxygen Therapy
title_sort markers of oxidant-antioxidant equilibrium in patients with sudden sensorineural hearing loss treated with hyperbaric oxygen therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381569/
https://www.ncbi.nlm.nih.gov/pubmed/30881599
http://dx.doi.org/10.1155/2019/8472346
work_keys_str_mv AT paprockijarosław markersofoxidantantioxidantequilibriuminpatientswithsuddensensorineuralhearinglosstreatedwithhyperbaricoxygentherapy
AT sutkowypaweł markersofoxidantantioxidantequilibriuminpatientswithsuddensensorineuralhearinglosstreatedwithhyperbaricoxygentherapy
AT piechockijacek markersofoxidantantioxidantequilibriuminpatientswithsuddensensorineuralhearinglosstreatedwithhyperbaricoxygentherapy
AT wozniakalina markersofoxidantantioxidantequilibriuminpatientswithsuddensensorineuralhearinglosstreatedwithhyperbaricoxygentherapy