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Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphorida...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382167/ https://www.ncbi.nlm.nih.gov/pubmed/30785945 http://dx.doi.org/10.1371/journal.pone.0212576 |
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author | Bedini, Stefano Flamini, Guido Cosci, Francesca Ascrizzi, Roberta Echeverria, Maria C. Gomez, Evelin V. Guidi, Lucia Landi, Marco Lucchi, Andrea Conti, Barbara |
author_facet | Bedini, Stefano Flamini, Guido Cosci, Francesca Ascrizzi, Roberta Echeverria, Maria C. Gomez, Evelin V. Guidi, Lucia Landi, Marco Lucchi, Andrea Conti, Barbara |
author_sort | Bedini, Stefano |
collection | PubMed |
description | Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphoridae) and compared with that of the well-known medicinal plant species Lavandula angustifolia Mill. The EOs were analysed and tested in laboratory for their oviposition deterrence and toxicity against L. sericata adults. The physiology of EO toxicity was evaluated by enzymatic inhibition tests. The antibacterial and antifungal properties of the EOs were tested as well. At 0.8 μL cm(-2), both EOs completely deterred L. sericata oviposition up to 3 hours. After 24 h, the oviposition deterrence was still 82.7% for L. angustifolia and the 89.5% for C. nubigenum. The two EOs were also toxic to eggs and adults of L. sericata. By contact/fumigation, the EOs, the LC(50) values against the eggs were 0.07 and 0.48 μL cm(-2) while, by topical application on the adults, LD(50) values were 0.278 and 0.393 μL per individual for C. nubigenum and L. angustifolia EOs, respectively. Inhibition of acetylcholine esterase of L. sericata by EOs (IC(50) = 67.450 and 79.495 mg L(-1) for C. nubigenum and L. angustifolia, respectively) suggested that the neural sites are targets of the EO toxicity. Finally, the observed antibacterial and antifungal properties of C. nubigenum and L. angustifolia EOs suggest that they could also help prevent secondary infections. |
format | Online Article Text |
id | pubmed-6382167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63821672019-03-01 Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata Bedini, Stefano Flamini, Guido Cosci, Francesca Ascrizzi, Roberta Echeverria, Maria C. Gomez, Evelin V. Guidi, Lucia Landi, Marco Lucchi, Andrea Conti, Barbara PLoS One Research Article Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphoridae) and compared with that of the well-known medicinal plant species Lavandula angustifolia Mill. The EOs were analysed and tested in laboratory for their oviposition deterrence and toxicity against L. sericata adults. The physiology of EO toxicity was evaluated by enzymatic inhibition tests. The antibacterial and antifungal properties of the EOs were tested as well. At 0.8 μL cm(-2), both EOs completely deterred L. sericata oviposition up to 3 hours. After 24 h, the oviposition deterrence was still 82.7% for L. angustifolia and the 89.5% for C. nubigenum. The two EOs were also toxic to eggs and adults of L. sericata. By contact/fumigation, the EOs, the LC(50) values against the eggs were 0.07 and 0.48 μL cm(-2) while, by topical application on the adults, LD(50) values were 0.278 and 0.393 μL per individual for C. nubigenum and L. angustifolia EOs, respectively. Inhibition of acetylcholine esterase of L. sericata by EOs (IC(50) = 67.450 and 79.495 mg L(-1) for C. nubigenum and L. angustifolia, respectively) suggested that the neural sites are targets of the EO toxicity. Finally, the observed antibacterial and antifungal properties of C. nubigenum and L. angustifolia EOs suggest that they could also help prevent secondary infections. Public Library of Science 2019-02-20 /pmc/articles/PMC6382167/ /pubmed/30785945 http://dx.doi.org/10.1371/journal.pone.0212576 Text en © 2019 Bedini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bedini, Stefano Flamini, Guido Cosci, Francesca Ascrizzi, Roberta Echeverria, Maria C. Gomez, Evelin V. Guidi, Lucia Landi, Marco Lucchi, Andrea Conti, Barbara Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title | Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title_full | Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title_fullStr | Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title_full_unstemmed | Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title_short | Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata |
title_sort | toxicity and oviposition deterrence of essential oils of clinopodium nubigenum and lavandula angustifolia against the myiasis-inducing blowfly lucilia sericata |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382167/ https://www.ncbi.nlm.nih.gov/pubmed/30785945 http://dx.doi.org/10.1371/journal.pone.0212576 |
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