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Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata

Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphorida...

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Autores principales: Bedini, Stefano, Flamini, Guido, Cosci, Francesca, Ascrizzi, Roberta, Echeverria, Maria C., Gomez, Evelin V., Guidi, Lucia, Landi, Marco, Lucchi, Andrea, Conti, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382167/
https://www.ncbi.nlm.nih.gov/pubmed/30785945
http://dx.doi.org/10.1371/journal.pone.0212576
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author Bedini, Stefano
Flamini, Guido
Cosci, Francesca
Ascrizzi, Roberta
Echeverria, Maria C.
Gomez, Evelin V.
Guidi, Lucia
Landi, Marco
Lucchi, Andrea
Conti, Barbara
author_facet Bedini, Stefano
Flamini, Guido
Cosci, Francesca
Ascrizzi, Roberta
Echeverria, Maria C.
Gomez, Evelin V.
Guidi, Lucia
Landi, Marco
Lucchi, Andrea
Conti, Barbara
author_sort Bedini, Stefano
collection PubMed
description Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphoridae) and compared with that of the well-known medicinal plant species Lavandula angustifolia Mill. The EOs were analysed and tested in laboratory for their oviposition deterrence and toxicity against L. sericata adults. The physiology of EO toxicity was evaluated by enzymatic inhibition tests. The antibacterial and antifungal properties of the EOs were tested as well. At 0.8 μL cm(-2), both EOs completely deterred L. sericata oviposition up to 3 hours. After 24 h, the oviposition deterrence was still 82.7% for L. angustifolia and the 89.5% for C. nubigenum. The two EOs were also toxic to eggs and adults of L. sericata. By contact/fumigation, the EOs, the LC(50) values against the eggs were 0.07 and 0.48 μL cm(-2) while, by topical application on the adults, LD(50) values were 0.278 and 0.393 μL per individual for C. nubigenum and L. angustifolia EOs, respectively. Inhibition of acetylcholine esterase of L. sericata by EOs (IC(50) = 67.450 and 79.495 mg L(-1) for C. nubigenum and L. angustifolia, respectively) suggested that the neural sites are targets of the EO toxicity. Finally, the observed antibacterial and antifungal properties of C. nubigenum and L. angustifolia EOs suggest that they could also help prevent secondary infections.
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spelling pubmed-63821672019-03-01 Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata Bedini, Stefano Flamini, Guido Cosci, Francesca Ascrizzi, Roberta Echeverria, Maria C. Gomez, Evelin V. Guidi, Lucia Landi, Marco Lucchi, Andrea Conti, Barbara PLoS One Research Article Cutaneous myiasis is a severe worldwide medical and veterinary issue. In this trial the essential oil (EO) of the Andean medicinal plant species Clinopodium nubigenum (Kunth) Kuntze was evaluated for its bioactivity against the myiasis-inducing blowfly Lucilia sericata (Meigen) (Diptera Calliphoridae) and compared with that of the well-known medicinal plant species Lavandula angustifolia Mill. The EOs were analysed and tested in laboratory for their oviposition deterrence and toxicity against L. sericata adults. The physiology of EO toxicity was evaluated by enzymatic inhibition tests. The antibacterial and antifungal properties of the EOs were tested as well. At 0.8 μL cm(-2), both EOs completely deterred L. sericata oviposition up to 3 hours. After 24 h, the oviposition deterrence was still 82.7% for L. angustifolia and the 89.5% for C. nubigenum. The two EOs were also toxic to eggs and adults of L. sericata. By contact/fumigation, the EOs, the LC(50) values against the eggs were 0.07 and 0.48 μL cm(-2) while, by topical application on the adults, LD(50) values were 0.278 and 0.393 μL per individual for C. nubigenum and L. angustifolia EOs, respectively. Inhibition of acetylcholine esterase of L. sericata by EOs (IC(50) = 67.450 and 79.495 mg L(-1) for C. nubigenum and L. angustifolia, respectively) suggested that the neural sites are targets of the EO toxicity. Finally, the observed antibacterial and antifungal properties of C. nubigenum and L. angustifolia EOs suggest that they could also help prevent secondary infections. Public Library of Science 2019-02-20 /pmc/articles/PMC6382167/ /pubmed/30785945 http://dx.doi.org/10.1371/journal.pone.0212576 Text en © 2019 Bedini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bedini, Stefano
Flamini, Guido
Cosci, Francesca
Ascrizzi, Roberta
Echeverria, Maria C.
Gomez, Evelin V.
Guidi, Lucia
Landi, Marco
Lucchi, Andrea
Conti, Barbara
Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title_full Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title_fullStr Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title_full_unstemmed Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title_short Toxicity and oviposition deterrence of essential oils of Clinopodium nubigenum and Lavandula angustifolia against the myiasis-inducing blowfly Lucilia sericata
title_sort toxicity and oviposition deterrence of essential oils of clinopodium nubigenum and lavandula angustifolia against the myiasis-inducing blowfly lucilia sericata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382167/
https://www.ncbi.nlm.nih.gov/pubmed/30785945
http://dx.doi.org/10.1371/journal.pone.0212576
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