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Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula

The number of legume root nodules resulting from a symbiosis with rhizobia is tightly controlled by the plant. Certain members of the CLAVATA3/Embryo Surrounding Region (CLE) peptide family, specifically MtCLE12 and MtCLE13 in Medicago truncatula, act in the systemic autoregulation of nodulation (AO...

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Autores principales: Gautrat, Pierre, Mortier, Virginie, Laffont, Carole, De Keyser, Annick, Fromentin, Justine, Frugier, Florian, Goormachtig, Sofie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382332/
https://www.ncbi.nlm.nih.gov/pubmed/30753553
http://dx.doi.org/10.1093/jxb/ery465
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author Gautrat, Pierre
Mortier, Virginie
Laffont, Carole
De Keyser, Annick
Fromentin, Justine
Frugier, Florian
Goormachtig, Sofie
author_facet Gautrat, Pierre
Mortier, Virginie
Laffont, Carole
De Keyser, Annick
Fromentin, Justine
Frugier, Florian
Goormachtig, Sofie
author_sort Gautrat, Pierre
collection PubMed
description The number of legume root nodules resulting from a symbiosis with rhizobia is tightly controlled by the plant. Certain members of the CLAVATA3/Embryo Surrounding Region (CLE) peptide family, specifically MtCLE12 and MtCLE13 in Medicago truncatula, act in the systemic autoregulation of nodulation (AON) pathway that negatively regulates the number of nodules. Little is known about the molecular pathways that operate downstream of the AON-related CLE peptides. Here, by means of a transcriptome analysis, we show that roots ectopically expressing MtCLE13 deregulate only a limited number of genes, including three down-regulated genes encoding lysin motif receptor-like kinases (LysM-RLKs), among which are the nodulation factor (NF) receptor NF Perception gene (NFP) and two up-regulated genes, MtTML1 and MtTML2, encoding Too Much Love (TML)-related Kelch-repeat containing F-box proteins. The observed deregulation was specific for the ectopic expression of nodulation-related MtCLE genes and depended on the Super Numeric Nodules (SUNN) AON RLK. Moreover, overexpression and silencing of these two MtTML genes demonstrated that they play a role in the negative regulation of nodule numbers. Hence, the identified MtTML genes are the functional counterpart of the Lotus japonicus TML gene shown to be central in the AON pathway. Additionally, we propose that the down-regulation of a subset of LysM-RLK-encoding genes, among which is NFP, might contribute to the restriction of further nodulation once the first nodules have been formed.
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spelling pubmed-63823322019-02-25 Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula Gautrat, Pierre Mortier, Virginie Laffont, Carole De Keyser, Annick Fromentin, Justine Frugier, Florian Goormachtig, Sofie J Exp Bot Research Papers The number of legume root nodules resulting from a symbiosis with rhizobia is tightly controlled by the plant. Certain members of the CLAVATA3/Embryo Surrounding Region (CLE) peptide family, specifically MtCLE12 and MtCLE13 in Medicago truncatula, act in the systemic autoregulation of nodulation (AON) pathway that negatively regulates the number of nodules. Little is known about the molecular pathways that operate downstream of the AON-related CLE peptides. Here, by means of a transcriptome analysis, we show that roots ectopically expressing MtCLE13 deregulate only a limited number of genes, including three down-regulated genes encoding lysin motif receptor-like kinases (LysM-RLKs), among which are the nodulation factor (NF) receptor NF Perception gene (NFP) and two up-regulated genes, MtTML1 and MtTML2, encoding Too Much Love (TML)-related Kelch-repeat containing F-box proteins. The observed deregulation was specific for the ectopic expression of nodulation-related MtCLE genes and depended on the Super Numeric Nodules (SUNN) AON RLK. Moreover, overexpression and silencing of these two MtTML genes demonstrated that they play a role in the negative regulation of nodule numbers. Hence, the identified MtTML genes are the functional counterpart of the Lotus japonicus TML gene shown to be central in the AON pathway. Additionally, we propose that the down-regulation of a subset of LysM-RLK-encoding genes, among which is NFP, might contribute to the restriction of further nodulation once the first nodules have been formed. Oxford University Press 2019-02-01 2019-02-08 /pmc/articles/PMC6382332/ /pubmed/30753553 http://dx.doi.org/10.1093/jxb/ery465 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Gautrat, Pierre
Mortier, Virginie
Laffont, Carole
De Keyser, Annick
Fromentin, Justine
Frugier, Florian
Goormachtig, Sofie
Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title_full Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title_fullStr Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title_full_unstemmed Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title_short Unraveling new molecular players involved in the autoregulation of nodulation in Medicago truncatula
title_sort unraveling new molecular players involved in the autoregulation of nodulation in medicago truncatula
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382332/
https://www.ncbi.nlm.nih.gov/pubmed/30753553
http://dx.doi.org/10.1093/jxb/ery465
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