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MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Express...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382419/ https://www.ncbi.nlm.nih.gov/pubmed/30737360 http://dx.doi.org/10.18632/aging.101791 |
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author | Zhao, Zhiguo Gao, Dan Ma, Tie Zhang, Liping |
author_facet | Zhao, Zhiguo Gao, Dan Ma, Tie Zhang, Liping |
author_sort | Zhao, Zhiguo |
collection | PubMed |
description | MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epidermal growth factor receptor (EGFR) were enhanced in tissues with low miR-141 expression but were reduced by miR-141 overexpression, and there was a significant negative correlation between EGFR and miR-141 levels in HNSCC tissues (P < 0.01). Luciferase assays confirmed that miR-141 targeted EGFR mRNA. In vitro, miR-141 inhibited the proliferation and migration of Cal-27 and FaDu HNSCC cells with corresponding decreases in CDK4 and MMP2. miR-141 also enhanced the incidence of apoptosis among the cells with a corresponding decrease in bcl-2. In BALB/c mice injected with FaDu HNSCC cells, miR-141 mitigated hepatic metastasis and inhibited expression of EGFR, CDK4, bcl-2 and MMP2. These results suggest that miR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling. MiR-141 thus appears to be a potentially useful therapeutic target in the treatment of HNSCC. |
format | Online Article Text |
id | pubmed-6382419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-63824192019-02-27 MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma Zhao, Zhiguo Gao, Dan Ma, Tie Zhang, Liping Aging (Albany NY) Research Paper MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epidermal growth factor receptor (EGFR) were enhanced in tissues with low miR-141 expression but were reduced by miR-141 overexpression, and there was a significant negative correlation between EGFR and miR-141 levels in HNSCC tissues (P < 0.01). Luciferase assays confirmed that miR-141 targeted EGFR mRNA. In vitro, miR-141 inhibited the proliferation and migration of Cal-27 and FaDu HNSCC cells with corresponding decreases in CDK4 and MMP2. miR-141 also enhanced the incidence of apoptosis among the cells with a corresponding decrease in bcl-2. In BALB/c mice injected with FaDu HNSCC cells, miR-141 mitigated hepatic metastasis and inhibited expression of EGFR, CDK4, bcl-2 and MMP2. These results suggest that miR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling. MiR-141 thus appears to be a potentially useful therapeutic target in the treatment of HNSCC. Impact Journals 2019-02-08 /pmc/articles/PMC6382419/ /pubmed/30737360 http://dx.doi.org/10.18632/aging.101791 Text en Copyright © 2019 Zhao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Zhao, Zhiguo Gao, Dan Ma, Tie Zhang, Liping MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title | MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title_full | MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title_fullStr | MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title_full_unstemmed | MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title_short | MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
title_sort | microrna-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382419/ https://www.ncbi.nlm.nih.gov/pubmed/30737360 http://dx.doi.org/10.18632/aging.101791 |
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