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MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma

MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Express...

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Autores principales: Zhao, Zhiguo, Gao, Dan, Ma, Tie, Zhang, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382419/
https://www.ncbi.nlm.nih.gov/pubmed/30737360
http://dx.doi.org/10.18632/aging.101791
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author Zhao, Zhiguo
Gao, Dan
Ma, Tie
Zhang, Liping
author_facet Zhao, Zhiguo
Gao, Dan
Ma, Tie
Zhang, Liping
author_sort Zhao, Zhiguo
collection PubMed
description MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epidermal growth factor receptor (EGFR) were enhanced in tissues with low miR-141 expression but were reduced by miR-141 overexpression, and there was a significant negative correlation between EGFR and miR-141 levels in HNSCC tissues (P < 0.01). Luciferase assays confirmed that miR-141 targeted EGFR mRNA. In vitro, miR-141 inhibited the proliferation and migration of Cal-27 and FaDu HNSCC cells with corresponding decreases in CDK4 and MMP2. miR-141 also enhanced the incidence of apoptosis among the cells with a corresponding decrease in bcl-2. In BALB/c mice injected with FaDu HNSCC cells, miR-141 mitigated hepatic metastasis and inhibited expression of EGFR, CDK4, bcl-2 and MMP2. These results suggest that miR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling. MiR-141 thus appears to be a potentially useful therapeutic target in the treatment of HNSCC.
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spelling pubmed-63824192019-02-27 MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma Zhao, Zhiguo Gao, Dan Ma, Tie Zhang, Liping Aging (Albany NY) Research Paper MicroRNAs (miRNAs) serve as regulatory factors in both healthy tissue and various cancers. Here, we used an miRNA microarray to screen for miRNAs differentially expressed between HNSCC and adjacent epithelial tissue. Among these, levels of miR-141 were significantly reduced in HNSCC tissues. Expression levels of epidermal growth factor receptor (EGFR) were enhanced in tissues with low miR-141 expression but were reduced by miR-141 overexpression, and there was a significant negative correlation between EGFR and miR-141 levels in HNSCC tissues (P < 0.01). Luciferase assays confirmed that miR-141 targeted EGFR mRNA. In vitro, miR-141 inhibited the proliferation and migration of Cal-27 and FaDu HNSCC cells with corresponding decreases in CDK4 and MMP2. miR-141 also enhanced the incidence of apoptosis among the cells with a corresponding decrease in bcl-2. In BALB/c mice injected with FaDu HNSCC cells, miR-141 mitigated hepatic metastasis and inhibited expression of EGFR, CDK4, bcl-2 and MMP2. These results suggest that miR-141 functions as a tumor suppressor in HNSCC and that it suppresses tumor growth and metastasis by suppressing EGFR signaling. MiR-141 thus appears to be a potentially useful therapeutic target in the treatment of HNSCC. Impact Journals 2019-02-08 /pmc/articles/PMC6382419/ /pubmed/30737360 http://dx.doi.org/10.18632/aging.101791 Text en Copyright © 2019 Zhao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhao, Zhiguo
Gao, Dan
Ma, Tie
Zhang, Liping
MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title_full MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title_fullStr MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title_full_unstemmed MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title_short MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
title_sort microrna-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382419/
https://www.ncbi.nlm.nih.gov/pubmed/30737360
http://dx.doi.org/10.18632/aging.101791
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