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Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss

BACKGROUND: Maternal–fetal tolerance plays a fundamental role in the maintenance of pregnancy. However, this immunological tolerance can be influenced by intrauterine infections. Human amniotic epithelial cells (hAECs) have immunomodulatory effects and respond to invading pathogens through expressin...

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Autores principales: Motedayyen, Hossein, Fathi, Farshid, Fasihi-Ramandi, Mahdi, Sabzghabaee, Ali Mohammad, Taheri, Ramezan Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383334/
https://www.ncbi.nlm.nih.gov/pubmed/30815014
http://dx.doi.org/10.4103/jrms.JRMS_463_18
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author Motedayyen, Hossein
Fathi, Farshid
Fasihi-Ramandi, Mahdi
Sabzghabaee, Ali Mohammad
Taheri, Ramezan Ali
author_facet Motedayyen, Hossein
Fathi, Farshid
Fasihi-Ramandi, Mahdi
Sabzghabaee, Ali Mohammad
Taheri, Ramezan Ali
author_sort Motedayyen, Hossein
collection PubMed
description BACKGROUND: Maternal–fetal tolerance plays a fundamental role in the maintenance of pregnancy. However, this immunological tolerance can be influenced by intrauterine infections. Human amniotic epithelial cells (hAECs) have immunomodulatory effects and respond to invading pathogens through expressing various toll-like receptors (TLRs). We hypothesize that bacteria or bacterial products affect the immunosuppressive effects of hAECs through TLR stimulation. Here, we investigated how a successful pregnancy can be threatened by TLR4 activation on hAECs on lipopolysaccharide (LPS) engagement. MATERIALS AND METHODS: hAECs were isolated from the amniotic membrane received from six healthy pregnant women. The immunophenotyping of hAECs was studied by flow cytometry. The isolated hAECs (4 × 10(5) cells/ml) were cultured in 24-well plates in the presence or absence of LPS (5 μg/ml). After 24, 48, and 72 h of incubation, the culture supernatants of hAECs were collected, and the levels of interleukin-5 (IL-5), IL-6, IL-1β, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1), and prostaglandin E2 (PGE2) were measured by enzyme-linked immunosorbent assay. RESULTS: TLR4 activation showed a stimulatory effect on TGF-β1 production of hAECs (P < 0.001–0.05). PGE2 production of LPS-stimulated hAECs was significantly increased (P < 0.01–0.05). Moreover, TLR4 could induce TNF-α and IL-1β production of hAECs (P < 0.0001–0.01), while this effect was not observed on IL-6 production of hAECs. The IL-5 was produced at a very low level in two culture supernatants of hAECs, in which its production was independent of LPS effect. CONCLUSION: TLR4 activation by bacterial components on hAECs may be a potential risk factor for pregnancy complications.
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spelling pubmed-63833342019-02-27 Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss Motedayyen, Hossein Fathi, Farshid Fasihi-Ramandi, Mahdi Sabzghabaee, Ali Mohammad Taheri, Ramezan Ali J Res Med Sci Original Article BACKGROUND: Maternal–fetal tolerance plays a fundamental role in the maintenance of pregnancy. However, this immunological tolerance can be influenced by intrauterine infections. Human amniotic epithelial cells (hAECs) have immunomodulatory effects and respond to invading pathogens through expressing various toll-like receptors (TLRs). We hypothesize that bacteria or bacterial products affect the immunosuppressive effects of hAECs through TLR stimulation. Here, we investigated how a successful pregnancy can be threatened by TLR4 activation on hAECs on lipopolysaccharide (LPS) engagement. MATERIALS AND METHODS: hAECs were isolated from the amniotic membrane received from six healthy pregnant women. The immunophenotyping of hAECs was studied by flow cytometry. The isolated hAECs (4 × 10(5) cells/ml) were cultured in 24-well plates in the presence or absence of LPS (5 μg/ml). After 24, 48, and 72 h of incubation, the culture supernatants of hAECs were collected, and the levels of interleukin-5 (IL-5), IL-6, IL-1β, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1), and prostaglandin E2 (PGE2) were measured by enzyme-linked immunosorbent assay. RESULTS: TLR4 activation showed a stimulatory effect on TGF-β1 production of hAECs (P < 0.001–0.05). PGE2 production of LPS-stimulated hAECs was significantly increased (P < 0.01–0.05). Moreover, TLR4 could induce TNF-α and IL-1β production of hAECs (P < 0.0001–0.01), while this effect was not observed on IL-6 production of hAECs. The IL-5 was produced at a very low level in two culture supernatants of hAECs, in which its production was independent of LPS effect. CONCLUSION: TLR4 activation by bacterial components on hAECs may be a potential risk factor for pregnancy complications. Medknow Publications & Media Pvt Ltd 2019-01-31 /pmc/articles/PMC6383334/ /pubmed/30815014 http://dx.doi.org/10.4103/jrms.JRMS_463_18 Text en Copyright: © 2019 Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Motedayyen, Hossein
Fathi, Farshid
Fasihi-Ramandi, Mahdi
Sabzghabaee, Ali Mohammad
Taheri, Ramezan Ali
Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title_full Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title_fullStr Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title_full_unstemmed Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title_short Toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
title_sort toll-like receptor 4 activation on human amniotic epithelial cells is a risk factor for pregnancy loss
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383334/
https://www.ncbi.nlm.nih.gov/pubmed/30815014
http://dx.doi.org/10.4103/jrms.JRMS_463_18
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