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Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvir...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383394/ https://www.ncbi.nlm.nih.gov/pubmed/30881462 http://dx.doi.org/10.1155/2019/5130152 |
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author | Zhang, Yu Liu, Shuyun Guo, Weimin Hao, Chunxiang Wang, Mingjie Li, Xu Zhang, Xueliang Chen, Mingxue Wang, Zehao Sui, Xiang Peng, Jiang Wang, Yu Lu, Shibi Guo, Quanyi |
author_facet | Zhang, Yu Liu, Shuyun Guo, Weimin Hao, Chunxiang Wang, Mingjie Li, Xu Zhang, Xueliang Chen, Mingxue Wang, Zehao Sui, Xiang Peng, Jiang Wang, Yu Lu, Shibi Guo, Quanyi |
author_sort | Zhang, Yu |
collection | PubMed |
description | Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvironment was constructed by primary articular cartilage cells (pACs) and acellular cartilage extracellular matrix- (ACECM-) oriented scaffold cocultured with human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJMSCs) in vitro. The coculture groups showed more affluent cartilage special matrix ingredients including collagen II and aggrecan based on the results of histological staining and western blotting and cut down as many pACs as possible. The RT-PCR and cell viability experiments also demonstrated that hWJMSCs were successfully induced to differentiate into chondrocytes when cultured in the simulated cartilage microenvironment, as confirmed by the significant upregulation of collagen II and aggrecan, while the cell proliferation activity of pACs was significantly improved by cell-cell interactions. Therefore, compared with monoculture and chondrogenic induction of inducers, coculture providing a simulated native articular microenvironment was a potential and temperate way to regulate the biological behaviors of pACs and hWJMSCs to regenerate the hyaline articular cartilage. |
format | Online Article Text |
id | pubmed-6383394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63833942019-03-17 Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro Zhang, Yu Liu, Shuyun Guo, Weimin Hao, Chunxiang Wang, Mingjie Li, Xu Zhang, Xueliang Chen, Mingxue Wang, Zehao Sui, Xiang Peng, Jiang Wang, Yu Lu, Shibi Guo, Quanyi Stem Cells Int Research Article Seed cells of articular cartilage tissue engineering face many obstacles in their application because of the dedifferentiation of chondrocytes or unstable chondrogenic differentiation status of pluripotent stem cells. To overcome mentioned dilemmas, a simulation of the articular cartilage microenvironment was constructed by primary articular cartilage cells (pACs) and acellular cartilage extracellular matrix- (ACECM-) oriented scaffold cocultured with human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hWJMSCs) in vitro. The coculture groups showed more affluent cartilage special matrix ingredients including collagen II and aggrecan based on the results of histological staining and western blotting and cut down as many pACs as possible. The RT-PCR and cell viability experiments also demonstrated that hWJMSCs were successfully induced to differentiate into chondrocytes when cultured in the simulated cartilage microenvironment, as confirmed by the significant upregulation of collagen II and aggrecan, while the cell proliferation activity of pACs was significantly improved by cell-cell interactions. Therefore, compared with monoculture and chondrogenic induction of inducers, coculture providing a simulated native articular microenvironment was a potential and temperate way to regulate the biological behaviors of pACs and hWJMSCs to regenerate the hyaline articular cartilage. Hindawi 2019-02-07 /pmc/articles/PMC6383394/ /pubmed/30881462 http://dx.doi.org/10.1155/2019/5130152 Text en Copyright © 2019 Yu Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Yu Liu, Shuyun Guo, Weimin Hao, Chunxiang Wang, Mingjie Li, Xu Zhang, Xueliang Chen, Mingxue Wang, Zehao Sui, Xiang Peng, Jiang Wang, Yu Lu, Shibi Guo, Quanyi Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro |
title | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
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title_full | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
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title_fullStr | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
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title_full_unstemmed | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
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title_short | Coculture of hWJMSCs and pACs in Oriented Scaffold Enhances Hyaline Cartilage Regeneration In Vitro
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title_sort | coculture of hwjmscs and pacs in oriented scaffold enhances hyaline cartilage regeneration in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383394/ https://www.ncbi.nlm.nih.gov/pubmed/30881462 http://dx.doi.org/10.1155/2019/5130152 |
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