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iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites

Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. When E. tenella infects a host cell, the host produces a corresponding change to deal with damage caused by this infection. To date, our knowledge on the mechanism of how the host cell res...

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Autores principales: Zhao, Zongping, Zhao, Qiping, Zhu, Shunhai, Huang, Bing, Lv, Ling, Chen, Ting, Yan, Ming, Han, Hongyu, Dong, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383524/
https://www.ncbi.nlm.nih.gov/pubmed/30789155
http://dx.doi.org/10.1051/parasite/2019009
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author Zhao, Zongping
Zhao, Qiping
Zhu, Shunhai
Huang, Bing
Lv, Ling
Chen, Ting
Yan, Ming
Han, Hongyu
Dong, Hui
author_facet Zhao, Zongping
Zhao, Qiping
Zhu, Shunhai
Huang, Bing
Lv, Ling
Chen, Ting
Yan, Ming
Han, Hongyu
Dong, Hui
author_sort Zhao, Zongping
collection PubMed
description Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. When E. tenella infects a host cell, the host produces a corresponding change to deal with damage caused by this infection. To date, our knowledge on the mechanism of how the host cell responds to E. tenella infection is highly limited at both the molecular and cellular levels. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS was used to screen the differentially expressed proteins (DEPs) in BHK-21 cells infected with E. tenella sporozoites for 24 h post infection. In total, 6139 non-redundant distinct proteins were identified and 195 of these were found to have a fold change ratio ≥1.3 or ≤0.7 and p < 0.05, including 151 up-regulated proteins and 44 down-regulated proteins. The reliability of the proteomic data was further validated with qPCR and western blot. Gene Ontology enrichment indicated that the up-regulated DEPs were mainly involved in binding and catalytic activity, whereas the down-regulated DEPs were catalytic activity and molecular function regulators. Furthermore, KEGG pathway analysis showed that the DEPs participated in the PI3K-Akt, chemokine, Ras, Wnt, and p53 signaling pathways and so on, and the up-regulated and down-regulated DEPs mainly related to the ribosome and mRNA surveillance pathway, respectively. The data in this study provide an important basis to further analyze E. tenella host cell interactions.
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spelling pubmed-63835242019-03-01 iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites Zhao, Zongping Zhao, Qiping Zhu, Shunhai Huang, Bing Lv, Ling Chen, Ting Yan, Ming Han, Hongyu Dong, Hui Parasite Research Article Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. When E. tenella infects a host cell, the host produces a corresponding change to deal with damage caused by this infection. To date, our knowledge on the mechanism of how the host cell responds to E. tenella infection is highly limited at both the molecular and cellular levels. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS was used to screen the differentially expressed proteins (DEPs) in BHK-21 cells infected with E. tenella sporozoites for 24 h post infection. In total, 6139 non-redundant distinct proteins were identified and 195 of these were found to have a fold change ratio ≥1.3 or ≤0.7 and p < 0.05, including 151 up-regulated proteins and 44 down-regulated proteins. The reliability of the proteomic data was further validated with qPCR and western blot. Gene Ontology enrichment indicated that the up-regulated DEPs were mainly involved in binding and catalytic activity, whereas the down-regulated DEPs were catalytic activity and molecular function regulators. Furthermore, KEGG pathway analysis showed that the DEPs participated in the PI3K-Akt, chemokine, Ras, Wnt, and p53 signaling pathways and so on, and the up-regulated and down-regulated DEPs mainly related to the ribosome and mRNA surveillance pathway, respectively. The data in this study provide an important basis to further analyze E. tenella host cell interactions. EDP Sciences 2019-02-20 /pmc/articles/PMC6383524/ /pubmed/30789155 http://dx.doi.org/10.1051/parasite/2019009 Text en © Z. Zhao et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Zongping
Zhao, Qiping
Zhu, Shunhai
Huang, Bing
Lv, Ling
Chen, Ting
Yan, Ming
Han, Hongyu
Dong, Hui
iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title_full iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title_fullStr iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title_full_unstemmed iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title_short iTRAQ-based comparative proteomic analysis of cells infected with Eimeria tenella sporozoites
title_sort itraq-based comparative proteomic analysis of cells infected with eimeria tenella sporozoites
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383524/
https://www.ncbi.nlm.nih.gov/pubmed/30789155
http://dx.doi.org/10.1051/parasite/2019009
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