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Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies
Post-staining of extracellular vesicles (EVs) with lipid-anchored fluorophores (LAFs) such as PKH67 is a widely used strategy for studying EVs but it is associated with several pitfalls. The pitfalls discussed in this commentary are related to LAF labelling of non-EV species due to (1) lipoprotein c...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Taylor & Francis
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383605/ https://www.ncbi.nlm.nih.gov/pubmed/30815239 http://dx.doi.org/10.1080/20013078.2019.1582237 |