ABC cloning: An efficient, simple, and rapid restriction/ligase-free method

DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...

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Detalles Bibliográficos
Autores principales: El Qaidi, Samir, Hardwidge, Philip R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384314/
https://www.ncbi.nlm.nih.gov/pubmed/30834197
http://dx.doi.org/10.1016/j.mex.2019.02.007

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384314/
https://www.ncbi.nlm.nih.gov/pubmed/30834197
http://dx.doi.org/10.1016/j.mex.2019.02.007

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