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ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives. Here we describe a PCR-based cloning method named ABC cloning. This m...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384314/ https://www.ncbi.nlm.nih.gov/pubmed/30834197 http://dx.doi.org/10.1016/j.mex.2019.02.007 |