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Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus

The aim of the study was to evaluate the influence of ectromelia virus (ECTV) infection on actin cytoskeleton rearrangement in immune cells, such as macrophages and dendritic cells (DCs). Using scanning electron and fluorescence microscopy analysis we observed the presence of long actin-based cellul...

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Autores principales: Szulc-Dąbrowska, Lidia, Gregorczyk-Zboroch, Karolina P., Struzik, Justyna, Wyżewski, Zbigniew, Ostrowska, Agnieszka, Toka, Felix N., Gieryńska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Polish Society of Experimental and Clinical Immunology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384431/
https://www.ncbi.nlm.nih.gov/pubmed/30799983
http://dx.doi.org/10.5114/ceji.2018.81352
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author Szulc-Dąbrowska, Lidia
Gregorczyk-Zboroch, Karolina P.
Struzik, Justyna
Wyżewski, Zbigniew
Ostrowska, Agnieszka
Toka, Felix N.
Gieryńska, Małgorzata
author_facet Szulc-Dąbrowska, Lidia
Gregorczyk-Zboroch, Karolina P.
Struzik, Justyna
Wyżewski, Zbigniew
Ostrowska, Agnieszka
Toka, Felix N.
Gieryńska, Małgorzata
author_sort Szulc-Dąbrowska, Lidia
collection PubMed
description The aim of the study was to evaluate the influence of ectromelia virus (ECTV) infection on actin cytoskeleton rearrangement in immune cells, such as macrophages and dendritic cells (DCs). Using scanning electron and fluorescence microscopy analysis we observed the presence of long actin-based cellular extensions, formed by both types of immune cells at later stages of infection with ECTV. Such extensions contained straight tubulin filaments and numerous punctuate mitochondria. Moreover, these long cellular projections extended to a certain length and formed convex structures termed “cytoplasmic packets”. These structures contained numerous viral particles and presumably were sites of progeny virions’ release via budding. Further, discrete mitochondria and separated tubulin filaments that formed a scaffold for accumulated mitochondria were visible within cytoplasmic packets. ECTV-induced long actin-based protrusions resemble “cytoplasmic corridors” and probably participate in virus dissemination. Our data demonstrate the incredible capacity for adaptation of ECTV to its natural host immune cells, in which it can survive, replicate and induce effective mechanisms for viral spread and dissemination.
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spelling pubmed-63844312019-02-22 Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus Szulc-Dąbrowska, Lidia Gregorczyk-Zboroch, Karolina P. Struzik, Justyna Wyżewski, Zbigniew Ostrowska, Agnieszka Toka, Felix N. Gieryńska, Małgorzata Cent Eur J Immunol Experimental Immunology The aim of the study was to evaluate the influence of ectromelia virus (ECTV) infection on actin cytoskeleton rearrangement in immune cells, such as macrophages and dendritic cells (DCs). Using scanning electron and fluorescence microscopy analysis we observed the presence of long actin-based cellular extensions, formed by both types of immune cells at later stages of infection with ECTV. Such extensions contained straight tubulin filaments and numerous punctuate mitochondria. Moreover, these long cellular projections extended to a certain length and formed convex structures termed “cytoplasmic packets”. These structures contained numerous viral particles and presumably were sites of progeny virions’ release via budding. Further, discrete mitochondria and separated tubulin filaments that formed a scaffold for accumulated mitochondria were visible within cytoplasmic packets. ECTV-induced long actin-based protrusions resemble “cytoplasmic corridors” and probably participate in virus dissemination. Our data demonstrate the incredible capacity for adaptation of ECTV to its natural host immune cells, in which it can survive, replicate and induce effective mechanisms for viral spread and dissemination. Polish Society of Experimental and Clinical Immunology 2018-12-31 2018 /pmc/articles/PMC6384431/ /pubmed/30799983 http://dx.doi.org/10.5114/ceji.2018.81352 Text en Copyright: © 2018 Polish Society of Experimental and Clinical Immunology http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Experimental Immunology
Szulc-Dąbrowska, Lidia
Gregorczyk-Zboroch, Karolina P.
Struzik, Justyna
Wyżewski, Zbigniew
Ostrowska, Agnieszka
Toka, Felix N.
Gieryńska, Małgorzata
Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title_full Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title_fullStr Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title_full_unstemmed Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title_short Long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
title_sort long actin-based cellular protrusions as novel evidence of the cytopathic effect induced in immune cells infected by the ectromelia virus
topic Experimental Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384431/
https://www.ncbi.nlm.nih.gov/pubmed/30799983
http://dx.doi.org/10.5114/ceji.2018.81352
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