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Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction

An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was...

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Autores principales: Leichtweis, Maria G., Pereira, Carla, Prieto, M.A., Barreiro, Maria Filomena, Baraldi, Ilton José, Barros, Lillian, Ferreira, Isabel C.F.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384548/
https://www.ncbi.nlm.nih.gov/pubmed/30764526
http://dx.doi.org/10.3390/molecules24030573
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author Leichtweis, Maria G.
Pereira, Carla
Prieto, M.A.
Barreiro, Maria Filomena
Baraldi, Ilton José
Barros, Lillian
Ferreira, Isabel C.F.R.
author_facet Leichtweis, Maria G.
Pereira, Carla
Prieto, M.A.
Barreiro, Maria Filomena
Baraldi, Ilton José
Barros, Lillian
Ferreira, Isabel C.F.R.
author_sort Leichtweis, Maria G.
collection PubMed
description An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was applied using the variables of time, temperature, and ethanol content, in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely, cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and the content of the identified anthocyanins. Ultrasound extraction was the most effective method at 5.00 ± 0.15 min, 400.00 ± 32.00 W, and 47.98% ± 2.88% of ethanol obtaining 68.60% ± 2.06% of extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g (epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp.
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spelling pubmed-63845482019-02-23 Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction Leichtweis, Maria G. Pereira, Carla Prieto, M.A. Barreiro, Maria Filomena Baraldi, Ilton José Barros, Lillian Ferreira, Isabel C.F.R. Molecules Article An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was applied using the variables of time, temperature, and ethanol content, in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely, cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and the content of the identified anthocyanins. Ultrasound extraction was the most effective method at 5.00 ± 0.15 min, 400.00 ± 32.00 W, and 47.98% ± 2.88% of ethanol obtaining 68.60% ± 2.06% of extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g (epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp. MDPI 2019-02-05 /pmc/articles/PMC6384548/ /pubmed/30764526 http://dx.doi.org/10.3390/molecules24030573 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leichtweis, Maria G.
Pereira, Carla
Prieto, M.A.
Barreiro, Maria Filomena
Baraldi, Ilton José
Barros, Lillian
Ferreira, Isabel C.F.R.
Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title_full Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title_fullStr Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title_full_unstemmed Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title_short Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit Epicarp: Comparative Study with Conventional Heat-Based Extraction
title_sort ultrasound as a rapid and low-cost extraction procedure to obtain anthocyanin-based colorants from prunus spinosa l. fruit epicarp: comparative study with conventional heat-based extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384548/
https://www.ncbi.nlm.nih.gov/pubmed/30764526
http://dx.doi.org/10.3390/molecules24030573
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