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Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS

Candida auris, a newly-emerging Candida species, is a serious global health threat due to its multi-drug resistant pattern, difficulty to diagnose, and the high mortality associated with its invasive and bloodstream infections. Unlike C. albicans, and C. dubliniensis which can form true hyphae, C. a...

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Autores principales: Semreen, Mohammad H., Soliman, Sameh S. M., Saeed, Balsam Q., Alqarihi, Abdullah, Uppuluri, Priya, Ibrahim, Ashraf S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384714/
https://www.ncbi.nlm.nih.gov/pubmed/30678308
http://dx.doi.org/10.3390/molecules24030399
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author Semreen, Mohammad H.
Soliman, Sameh S. M.
Saeed, Balsam Q.
Alqarihi, Abdullah
Uppuluri, Priya
Ibrahim, Ashraf S.
author_facet Semreen, Mohammad H.
Soliman, Sameh S. M.
Saeed, Balsam Q.
Alqarihi, Abdullah
Uppuluri, Priya
Ibrahim, Ashraf S.
author_sort Semreen, Mohammad H.
collection PubMed
description Candida auris, a newly-emerging Candida species, is a serious global health threat due to its multi-drug resistant pattern, difficulty to diagnose, and the high mortality associated with its invasive and bloodstream infections. Unlike C. albicans, and C. dubliniensis which can form true hyphae, C. auris grows as yeast or pseudohyphae and is capable of developing biofilms. The reasons for the inability of C. auris to form true hyphae are currently unknown. Metabolites secreted by microorganisms, including Candida, are known as important factors in controlling morphogenesis and pathogenesis. Metabolic profiling of C. auris and C. albicans cultures was performed using gas chromatography–mass spectrometry (GC–MS). Compared to C. albicans, C. auris secreted several hyphae-inhibiting metabolites, including phenylethyl, benzyl and isoamyl alcohols. Furthermore, a biofilm-forming metabolite—tyrosol—was identified. On the other hand, several other biomarkers identified from C. auris but not from C. albicans cultures may be produced by the organism to overcome the host immune system or control fungal adaptations, and hence ease its invasion and infections. The results from this study are considered as the first identification of C. auris metabolic activities as a step forward to understand its virulence mechanisms.
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spelling pubmed-63847142019-02-23 Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS Semreen, Mohammad H. Soliman, Sameh S. M. Saeed, Balsam Q. Alqarihi, Abdullah Uppuluri, Priya Ibrahim, Ashraf S. Molecules Article Candida auris, a newly-emerging Candida species, is a serious global health threat due to its multi-drug resistant pattern, difficulty to diagnose, and the high mortality associated with its invasive and bloodstream infections. Unlike C. albicans, and C. dubliniensis which can form true hyphae, C. auris grows as yeast or pseudohyphae and is capable of developing biofilms. The reasons for the inability of C. auris to form true hyphae are currently unknown. Metabolites secreted by microorganisms, including Candida, are known as important factors in controlling morphogenesis and pathogenesis. Metabolic profiling of C. auris and C. albicans cultures was performed using gas chromatography–mass spectrometry (GC–MS). Compared to C. albicans, C. auris secreted several hyphae-inhibiting metabolites, including phenylethyl, benzyl and isoamyl alcohols. Furthermore, a biofilm-forming metabolite—tyrosol—was identified. On the other hand, several other biomarkers identified from C. auris but not from C. albicans cultures may be produced by the organism to overcome the host immune system or control fungal adaptations, and hence ease its invasion and infections. The results from this study are considered as the first identification of C. auris metabolic activities as a step forward to understand its virulence mechanisms. MDPI 2019-01-22 /pmc/articles/PMC6384714/ /pubmed/30678308 http://dx.doi.org/10.3390/molecules24030399 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Semreen, Mohammad H.
Soliman, Sameh S. M.
Saeed, Balsam Q.
Alqarihi, Abdullah
Uppuluri, Priya
Ibrahim, Ashraf S.
Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title_full Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title_fullStr Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title_full_unstemmed Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title_short Metabolic Profiling of Candida auris, a Newly-Emerging Multi-Drug Resistant Candida Species, by GC-MS
title_sort metabolic profiling of candida auris, a newly-emerging multi-drug resistant candida species, by gc-ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384714/
https://www.ncbi.nlm.nih.gov/pubmed/30678308
http://dx.doi.org/10.3390/molecules24030399
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