A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells

A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhi...

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Detalles Bibliográficos
Autores principales: Ma, Xiaohua, Hao, Yuanqiang, Liu, Jiaxiang, Wu, Guoguang, Liu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384771/
https://www.ncbi.nlm.nih.gov/pubmed/30678112
http://dx.doi.org/10.3390/molecules24030411
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author Ma, Xiaohua
Hao, Yuanqiang
Liu, Jiaxiang
Wu, Guoguang
Liu, Lin
author_facet Ma, Xiaohua
Hao, Yuanqiang
Liu, Jiaxiang
Wu, Guoguang
Liu, Lin
author_sort Ma, Xiaohua
collection PubMed
description A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells.
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spelling pubmed-63847712019-02-23 A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells Ma, Xiaohua Hao, Yuanqiang Liu, Jiaxiang Wu, Guoguang Liu, Lin Molecules Article A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells. MDPI 2019-01-23 /pmc/articles/PMC6384771/ /pubmed/30678112 http://dx.doi.org/10.3390/molecules24030411 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Xiaohua
Hao, Yuanqiang
Liu, Jiaxiang
Wu, Guoguang
Liu, Lin
A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title_full A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title_fullStr A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title_full_unstemmed A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title_short A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells
title_sort green-emitting fluorescent probe based on a benzothiazole derivative for imaging biothiols in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384771/
https://www.ncbi.nlm.nih.gov/pubmed/30678112
http://dx.doi.org/10.3390/molecules24030411
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