QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices

A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by H...

Descripción completa

Detalles Bibliográficos
Autores principales: Śniegocki, Tomasz, Sell, Bartosz, Giergiel, Marta, Posyniak, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384814/
https://www.ncbi.nlm.nih.gov/pubmed/30678224
http://dx.doi.org/10.3390/molecules24030384
Descripción
Sumario:A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by HPLC-MS/MS in negative electrospray mode by monitoring the daughter ions m/z: 321→194 and 321→152. The limit of decision (CCα) was calculated at the range of 0.10 μg kg(−1) to 0.15 μg kg(−1) and detection capability (CCβ) from 0.12 μg kg(−1) to 0.18 μg kg(−1). Validation results showed that this method is suitable for the determination and confirmation of chloramphenicol in various matrices.

Ejemplares similares