QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices

A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by H...

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Autores principales: Śniegocki, Tomasz, Sell, Bartosz, Giergiel, Marta, Posyniak, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384814/
https://www.ncbi.nlm.nih.gov/pubmed/30678224
http://dx.doi.org/10.3390/molecules24030384
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author Śniegocki, Tomasz
Sell, Bartosz
Giergiel, Marta
Posyniak, Andrzej
author_facet Śniegocki, Tomasz
Sell, Bartosz
Giergiel, Marta
Posyniak, Andrzej
author_sort Śniegocki, Tomasz
collection PubMed
description A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by HPLC-MS/MS in negative electrospray mode by monitoring the daughter ions m/z: 321→194 and 321→152. The limit of decision (CCα) was calculated at the range of 0.10 μg kg(−1) to 0.15 μg kg(−1) and detection capability (CCβ) from 0.12 μg kg(−1) to 0.18 μg kg(−1). Validation results showed that this method is suitable for the determination and confirmation of chloramphenicol in various matrices.
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spelling pubmed-63848142019-02-23 QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices Śniegocki, Tomasz Sell, Bartosz Giergiel, Marta Posyniak, Andrzej Molecules Article A simple method for the determination of chloramphenicol in 22 matrices was prepared based on the QuEChERS and HPLC-MS/MS combination. Following a hydrolysis step, the homogenized samples were extracted and partitioned after adding sodium chloride with acetonitrile. Chloramphenicol was analysed by HPLC-MS/MS in negative electrospray mode by monitoring the daughter ions m/z: 321→194 and 321→152. The limit of decision (CCα) was calculated at the range of 0.10 μg kg(−1) to 0.15 μg kg(−1) and detection capability (CCβ) from 0.12 μg kg(−1) to 0.18 μg kg(−1). Validation results showed that this method is suitable for the determination and confirmation of chloramphenicol in various matrices. MDPI 2019-01-22 /pmc/articles/PMC6384814/ /pubmed/30678224 http://dx.doi.org/10.3390/molecules24030384 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Śniegocki, Tomasz
Sell, Bartosz
Giergiel, Marta
Posyniak, Andrzej
QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title_full QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title_fullStr QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title_full_unstemmed QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title_short QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty Two Different Matrices
title_sort quechers and hplc-ms/ms combination for the determination of chloramphenicol in twenty two different matrices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384814/
https://www.ncbi.nlm.nih.gov/pubmed/30678224
http://dx.doi.org/10.3390/molecules24030384
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