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Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells

The antibiotic resistance of Salmonella has become increasingly serious due to the increased use of antibiotics, and antimicrobial peptides have been considered as an ideal antibiotic alternative. Salmonella can induce macrophage apoptosis and thus further damage the immune system. The antimicrobial...

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Autores principales: Wang, Lei, Zhao, Xueqin, Xia, Xiaojing, Zhu, Chunling, Zhang, Huihui, Qin, Wanhai, Xu, Yanzhao, Hang, Bolin, Sun, Yawei, Chen, Shijun, Jiang, Jinqing, Zhang, Gaiping, Hu, Jianhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384860/
https://www.ncbi.nlm.nih.gov/pubmed/30736473
http://dx.doi.org/10.3390/molecules24030596
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author Wang, Lei
Zhao, Xueqin
Xia, Xiaojing
Zhu, Chunling
Zhang, Huihui
Qin, Wanhai
Xu, Yanzhao
Hang, Bolin
Sun, Yawei
Chen, Shijun
Jiang, Jinqing
Zhang, Gaiping
Hu, Jianhe
author_facet Wang, Lei
Zhao, Xueqin
Xia, Xiaojing
Zhu, Chunling
Zhang, Huihui
Qin, Wanhai
Xu, Yanzhao
Hang, Bolin
Sun, Yawei
Chen, Shijun
Jiang, Jinqing
Zhang, Gaiping
Hu, Jianhe
author_sort Wang, Lei
collection PubMed
description The antibiotic resistance of Salmonella has become increasingly serious due to the increased use of antibiotics, and antimicrobial peptides have been considered as an ideal antibiotic alternative. Salmonella can induce macrophage apoptosis and thus further damage the immune system. The antimicrobial peptide JH-3 has been shown to have a satisfactory anti-Salmonella effect in previous research, but its mechanism of action remains unknown. In this study, the effects of JH-3 on macrophages infected with Salmonella Typhimurium CVCC541 were evaluated at the cellular level. The results showed that JH-3 significantly alleviated the damage to macrophages caused by S. Typhi infection, reduced the release of lactic dehydrogenase (LDH), and killed the bacteria in macrophages. In addition, JH-3 decreased the phosphorylation level of p65 and the expression and secretion of interleukin 2 (IL-2), IL-6, and tumor necrosis factor-α (TNF-α) by inhibiting the activation of the mitogen-activated protein kinase (MAPK) (p38) signaling pathway and alleviating the cellular inflammatory response. From confocal laser scanning microscopy and flow cytometry assays, JH-3 was observed to inhibit the release of cytochrome c in the cytoplasm; the expression of TNF-αR2, caspase-9, and caspase-8; to further weaken caspase-3 activation; and to reduce the S.-Typhi-induced apoptosis of macrophages. In summary, the mechanism by which JH-3 inhibits Salmonella infection was systematically explored at the cellular level, laying the foundation for the development and utilization of JH-3 as a therapeutic alternative to antibiotics.
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spelling pubmed-63848602019-02-23 Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells Wang, Lei Zhao, Xueqin Xia, Xiaojing Zhu, Chunling Zhang, Huihui Qin, Wanhai Xu, Yanzhao Hang, Bolin Sun, Yawei Chen, Shijun Jiang, Jinqing Zhang, Gaiping Hu, Jianhe Molecules Article The antibiotic resistance of Salmonella has become increasingly serious due to the increased use of antibiotics, and antimicrobial peptides have been considered as an ideal antibiotic alternative. Salmonella can induce macrophage apoptosis and thus further damage the immune system. The antimicrobial peptide JH-3 has been shown to have a satisfactory anti-Salmonella effect in previous research, but its mechanism of action remains unknown. In this study, the effects of JH-3 on macrophages infected with Salmonella Typhimurium CVCC541 were evaluated at the cellular level. The results showed that JH-3 significantly alleviated the damage to macrophages caused by S. Typhi infection, reduced the release of lactic dehydrogenase (LDH), and killed the bacteria in macrophages. In addition, JH-3 decreased the phosphorylation level of p65 and the expression and secretion of interleukin 2 (IL-2), IL-6, and tumor necrosis factor-α (TNF-α) by inhibiting the activation of the mitogen-activated protein kinase (MAPK) (p38) signaling pathway and alleviating the cellular inflammatory response. From confocal laser scanning microscopy and flow cytometry assays, JH-3 was observed to inhibit the release of cytochrome c in the cytoplasm; the expression of TNF-αR2, caspase-9, and caspase-8; to further weaken caspase-3 activation; and to reduce the S.-Typhi-induced apoptosis of macrophages. In summary, the mechanism by which JH-3 inhibits Salmonella infection was systematically explored at the cellular level, laying the foundation for the development and utilization of JH-3 as a therapeutic alternative to antibiotics. MDPI 2019-02-07 /pmc/articles/PMC6384860/ /pubmed/30736473 http://dx.doi.org/10.3390/molecules24030596 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Lei
Zhao, Xueqin
Xia, Xiaojing
Zhu, Chunling
Zhang, Huihui
Qin, Wanhai
Xu, Yanzhao
Hang, Bolin
Sun, Yawei
Chen, Shijun
Jiang, Jinqing
Zhang, Gaiping
Hu, Jianhe
Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title_full Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title_fullStr Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title_full_unstemmed Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title_short Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells
title_sort inhibitory effects of antimicrobial peptide jh-3 on salmonella enterica serovar typhimurium strain cvcc541 infection-induced inflammatory cytokine release and apoptosis in raw264.7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384860/
https://www.ncbi.nlm.nih.gov/pubmed/30736473
http://dx.doi.org/10.3390/molecules24030596
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