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Photoacoustic transfection of DNA encoding GFP

Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show...

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Detalles Bibliográficos
Autores principales: Silva, Alexandre D., Serpa, Carlos, Arnaut, Luis G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384874/
https://www.ncbi.nlm.nih.gov/pubmed/30796229
http://dx.doi.org/10.1038/s41598-018-37759-1
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author Silva, Alexandre D.
Serpa, Carlos
Arnaut, Luis G.
author_facet Silva, Alexandre D.
Serpa, Carlos
Arnaut, Luis G.
author_sort Silva, Alexandre D.
collection PubMed
description Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ/cm(2) are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green Fluorescent Protein (gWizGFP, 3.74 MDa) in COS-7 monkey fibroblast cells with an efficiency of 5% at 20 °C, in 10 minutes. We did not observe significant cytotoxicity under these conditions. Photoacoustic transfection is scalable, affordable, enables nuclear localization and the dosage is easily controlled by the laser parameters.
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spelling pubmed-63848742019-02-26 Photoacoustic transfection of DNA encoding GFP Silva, Alexandre D. Serpa, Carlos Arnaut, Luis G. Sci Rep Article Photoacoustic transfection consists in the use of photoacoustic waves, generated in the thermoelastic expansion of a confined material absorbing a short pulse of a laser, to produce temporary mechanical deformations of the cell membrane and facilitate the delivery of plasmid DNA into cells. We show that high stress gradients, produced when picosecond laser pulses with a fluence of 100 mJ/cm(2) are absorbed by piezophotonic materials, enable transfection of a plasmid DNA encoding Green Fluorescent Protein (gWizGFP, 3.74 MDa) in COS-7 monkey fibroblast cells with an efficiency of 5% at 20 °C, in 10 minutes. We did not observe significant cytotoxicity under these conditions. Photoacoustic transfection is scalable, affordable, enables nuclear localization and the dosage is easily controlled by the laser parameters. Nature Publishing Group UK 2019-02-22 /pmc/articles/PMC6384874/ /pubmed/30796229 http://dx.doi.org/10.1038/s41598-018-37759-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Silva, Alexandre D.
Serpa, Carlos
Arnaut, Luis G.
Photoacoustic transfection of DNA encoding GFP
title Photoacoustic transfection of DNA encoding GFP
title_full Photoacoustic transfection of DNA encoding GFP
title_fullStr Photoacoustic transfection of DNA encoding GFP
title_full_unstemmed Photoacoustic transfection of DNA encoding GFP
title_short Photoacoustic transfection of DNA encoding GFP
title_sort photoacoustic transfection of dna encoding gfp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384874/
https://www.ncbi.nlm.nih.gov/pubmed/30796229
http://dx.doi.org/10.1038/s41598-018-37759-1
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