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HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris

Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and...

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Autores principales: Taddeo, Vito Alessandro, Epifano, Francesco, Preziuso, Francesca, Fiorito, Serena, Caron, Nicolas, Rives, Arnaud, de Medina, Philippe, Poirot, Marc, Silvente-Poirot, Sandrine, Genovese, Salvatore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384930/
https://www.ncbi.nlm.nih.gov/pubmed/30704124
http://dx.doi.org/10.3390/molecules24030501
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author Taddeo, Vito Alessandro
Epifano, Francesco
Preziuso, Francesca
Fiorito, Serena
Caron, Nicolas
Rives, Arnaud
de Medina, Philippe
Poirot, Marc
Silvente-Poirot, Sandrine
Genovese, Salvatore
author_facet Taddeo, Vito Alessandro
Epifano, Francesco
Preziuso, Francesca
Fiorito, Serena
Caron, Nicolas
Rives, Arnaud
de Medina, Philippe
Poirot, Marc
Silvente-Poirot, Sandrine
Genovese, Salvatore
author_sort Taddeo, Vito Alessandro
collection PubMed
description Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and Ferulago campestris (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of F. campestris as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., A. graveolens).
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spelling pubmed-63849302019-02-23 HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris Taddeo, Vito Alessandro Epifano, Francesco Preziuso, Francesca Fiorito, Serena Caron, Nicolas Rives, Arnaud de Medina, Philippe Poirot, Marc Silvente-Poirot, Sandrine Genovese, Salvatore Molecules Communication Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and Ferulago campestris (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of F. campestris as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., A. graveolens). MDPI 2019-01-30 /pmc/articles/PMC6384930/ /pubmed/30704124 http://dx.doi.org/10.3390/molecules24030501 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Taddeo, Vito Alessandro
Epifano, Francesco
Preziuso, Francesca
Fiorito, Serena
Caron, Nicolas
Rives, Arnaud
de Medina, Philippe
Poirot, Marc
Silvente-Poirot, Sandrine
Genovese, Salvatore
HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title_full HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title_fullStr HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title_full_unstemmed HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title_short HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris
title_sort hplc analysis and skin whitening effects of umbelliprenin-containing extracts of anethum graveolens, pimpinella anisum, and ferulago campestris
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384930/
https://www.ncbi.nlm.nih.gov/pubmed/30704124
http://dx.doi.org/10.3390/molecules24030501
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