New Anti-inflammatory Flavonol Glycosides from Lindera akoensis Hayata

Inflammation is related to many diseases. Lindera akoensis Hayata was often used in folk therapy in Taiwan for inflammation. In this study, three new flavonol acyl glycosides, namely kaempferol-3-O-β-D-4′′,6′′-di-(E)-p-coumaroylglucoside (1), 3′′-(E)-p-coumaroylafzelin (2) and 4′-O- methyl-2′′,4′′-di-(E)-p-coumaroylquercitrin (3), and three components, 3β-dodecyl-4β-hydroxy- 5β-methyldihydrofuran-2-one (4), 2β-acetoxyclovan-9α-ol (5), (1α,4β,6β)-trihydroxyeudesmane (6) that were isolated from the natural product for the first time were obtained along with 25 known compounds from L. akoensis. Their structures were determined by comprehensive spectroscopic analyses (1D and 2D NMR, EI-, ESI- and HRESI-MS). The ability of 1 to decrease the LPS-stimulated production of nitrite in RAW264.7 cell was evaluated, showing an IC(50) value of 36.3 ± 3.2 μM. This result supports the value of L. akoensis as a traditional medicine resource.