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Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet
Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). True hybrid progenies were precisely identified u...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385618/ https://www.ncbi.nlm.nih.gov/pubmed/30792411 http://dx.doi.org/10.1038/s41598-019-38710-8 |
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author | Yang, Shan Zeng, Kai Chen, Ke Wu, Jiayun Wang, Qinnan Li, Xueting Deng, Zuhu Huang, Yongji Huang, Fei Chen, Rukai Zhang, Muqing |
author_facet | Yang, Shan Zeng, Kai Chen, Ke Wu, Jiayun Wang, Qinnan Li, Xueting Deng, Zuhu Huang, Yongji Huang, Fei Chen, Rukai Zhang, Muqing |
author_sort | Yang, Shan |
collection | PubMed |
description | Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). True hybrid progenies were precisely identified using PCR with a primer pair, AGRP52/53. The results showed that AGRP52/53 was an E. arundinaceus-specific primer pair and could be used as molecular marker to assist breeding. EaHN92, a 364 bp E. arundinaceus-specific tandem repeat satellite DNA sequence, was cloned from the E. arundinaceus clone HN92–105 with AGRP52/53, and was localized on sub-telomeric regions of all E. arundinaceus chromosomes. YCE06–61, a BC(3) progeny, had 7 E. arundinaceus chromosomes and its progenies had approximately 1–6 E. arundinaceus chromosomes. The number of E. arundinaceus chromosomes in true hybrids appeared as Gaussian distribution in 3 cross combinations. In addition, GISH detected intergeneric chromosome translocation in a few progenies. Hence, screening clones containing approximately 1–2 E. arundinaceus chromosomes without translocation could be used for sorting and sequencing E. arundinaceus chromosomes. This study provides a method for breeders to select true hybrid progenies between Saccharum spp. and E. arundinaceus, which will accelerate this intergeneric hybridization breeding. |
format | Online Article Text |
id | pubmed-6385618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63856182019-02-27 Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet Yang, Shan Zeng, Kai Chen, Ke Wu, Jiayun Wang, Qinnan Li, Xueting Deng, Zuhu Huang, Yongji Huang, Fei Chen, Rukai Zhang, Muqing Sci Rep Article Intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus and clones derived from these hybrids and backcrosses to Saccharum spp. were used to study the transmission of E. arundinaceus chromosomes by genomic in situ hybridization (GISH). True hybrid progenies were precisely identified using PCR with a primer pair, AGRP52/53. The results showed that AGRP52/53 was an E. arundinaceus-specific primer pair and could be used as molecular marker to assist breeding. EaHN92, a 364 bp E. arundinaceus-specific tandem repeat satellite DNA sequence, was cloned from the E. arundinaceus clone HN92–105 with AGRP52/53, and was localized on sub-telomeric regions of all E. arundinaceus chromosomes. YCE06–61, a BC(3) progeny, had 7 E. arundinaceus chromosomes and its progenies had approximately 1–6 E. arundinaceus chromosomes. The number of E. arundinaceus chromosomes in true hybrids appeared as Gaussian distribution in 3 cross combinations. In addition, GISH detected intergeneric chromosome translocation in a few progenies. Hence, screening clones containing approximately 1–2 E. arundinaceus chromosomes without translocation could be used for sorting and sequencing E. arundinaceus chromosomes. This study provides a method for breeders to select true hybrid progenies between Saccharum spp. and E. arundinaceus, which will accelerate this intergeneric hybridization breeding. Nature Publishing Group UK 2019-02-21 /pmc/articles/PMC6385618/ /pubmed/30792411 http://dx.doi.org/10.1038/s41598-019-38710-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Shan Zeng, Kai Chen, Ke Wu, Jiayun Wang, Qinnan Li, Xueting Deng, Zuhu Huang, Yongji Huang, Fei Chen, Rukai Zhang, Muqing Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title | Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title_full | Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title_fullStr | Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title_full_unstemmed | Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title_short | Chromosome transmission in BC(4) progenies of intergeneric hybrids between Saccharum spp. and Erianthus arundinaceus (Retz.) Jeswiet |
title_sort | chromosome transmission in bc(4) progenies of intergeneric hybrids between saccharum spp. and erianthus arundinaceus (retz.) jeswiet |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385618/ https://www.ncbi.nlm.nih.gov/pubmed/30792411 http://dx.doi.org/10.1038/s41598-019-38710-8 |
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