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Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP
A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (10(4) and 10(5) spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Plant Pathology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385655/ https://www.ncbi.nlm.nih.gov/pubmed/30828279 http://dx.doi.org/10.5423/PPJ.OA.11.2017.0253 |
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author | Kamran, Muhammad Javed, Nazir Ullah, Ihsan Nazir, Shahid Jamil, Shakra Iqbal, Muhammad Zafar Abbas, Huma Khan, Sajid Aleem Ehetisham ul Haq, Muhammad |
author_facet | Kamran, Muhammad Javed, Nazir Ullah, Ihsan Nazir, Shahid Jamil, Shakra Iqbal, Muhammad Zafar Abbas, Huma Khan, Sajid Aleem Ehetisham ul Haq, Muhammad |
author_sort | Kamran, Muhammad |
collection | PubMed |
description | A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (10(4) and 10(5) spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans. |
format | Online Article Text |
id | pubmed-6385655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-63856552019-03-01 Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP Kamran, Muhammad Javed, Nazir Ullah, Ihsan Nazir, Shahid Jamil, Shakra Iqbal, Muhammad Zafar Abbas, Huma Khan, Sajid Aleem Ehetisham ul Haq, Muhammad Plant Pathol J Research Article A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (10(4) and 10(5) spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans. Korean Society of Plant Pathology 2019-02 2019-02-01 /pmc/articles/PMC6385655/ /pubmed/30828279 http://dx.doi.org/10.5423/PPJ.OA.11.2017.0253 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kamran, Muhammad Javed, Nazir Ullah, Ihsan Nazir, Shahid Jamil, Shakra Iqbal, Muhammad Zafar Abbas, Huma Khan, Sajid Aleem Ehetisham ul Haq, Muhammad Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title | Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title_full | Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title_fullStr | Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title_full_unstemmed | Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title_short | Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP |
title_sort | genetic variability among different populations of root knot nematodes based on their encumbrance response to pasteuria isolates using pcr-rflp |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385655/ https://www.ncbi.nlm.nih.gov/pubmed/30828279 http://dx.doi.org/10.5423/PPJ.OA.11.2017.0253 |
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