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GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and ph...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385990/ https://www.ncbi.nlm.nih.gov/pubmed/30530644 http://dx.doi.org/10.1534/g3.118.200809 |
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author | Pérez-Moreno, Juan J. O’Kane, Cahir J. |
author_facet | Pérez-Moreno, Juan J. O’Kane, Cahir J. |
author_sort | Pérez-Moreno, Juan J. |
collection | PubMed |
description | The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons. |
format | Online Article Text |
id | pubmed-6385990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-63859902019-02-26 GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila Pérez-Moreno, Juan J. O’Kane, Cahir J. G3 (Bethesda) Investigations The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons. Genetics Society of America 2018-12-10 /pmc/articles/PMC6385990/ /pubmed/30530644 http://dx.doi.org/10.1534/g3.118.200809 Text en Copyright © 2019 Pérez-Moreno, O’Kane http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Pérez-Moreno, Juan J. O’Kane, Cahir J. GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title | GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title_full | GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title_fullStr | GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title_full_unstemmed | GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title_short | GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila |
title_sort | gal4 drivers specific for type ib and type is motor neurons in drosophila |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385990/ https://www.ncbi.nlm.nih.gov/pubmed/30530644 http://dx.doi.org/10.1534/g3.118.200809 |
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