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GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila

The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and ph...

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Autores principales: Pérez-Moreno, Juan J., O’Kane, Cahir J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385990/
https://www.ncbi.nlm.nih.gov/pubmed/30530644
http://dx.doi.org/10.1534/g3.118.200809
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author Pérez-Moreno, Juan J.
O’Kane, Cahir J.
author_facet Pérez-Moreno, Juan J.
O’Kane, Cahir J.
author_sort Pérez-Moreno, Juan J.
collection PubMed
description The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons.
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spelling pubmed-63859902019-02-26 GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila Pérez-Moreno, Juan J. O’Kane, Cahir J. G3 (Bethesda) Investigations The Drosophila melanogaster larval neuromuscular system is extensively used by researchers to study neuronal cell biology, and Drosophila glutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened for GAL4 driver lines for this purpose. Here we describe GAL4 drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons. Genetics Society of America 2018-12-10 /pmc/articles/PMC6385990/ /pubmed/30530644 http://dx.doi.org/10.1534/g3.118.200809 Text en Copyright © 2019 Pérez-Moreno, O’Kane http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Pérez-Moreno, Juan J.
O’Kane, Cahir J.
GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title_full GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title_fullStr GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title_full_unstemmed GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title_short GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila
title_sort gal4 drivers specific for type ib and type is motor neurons in drosophila
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385990/
https://www.ncbi.nlm.nih.gov/pubmed/30530644
http://dx.doi.org/10.1534/g3.118.200809
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