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Effect of PTEN loss on metabolic reprogramming in prostate cancer cells
The tumor suppressor gene PTEN is one of the most often deleted genes in human prostate cancer. Loss of PTEN is an important event in prostate carcinogenesis. Metabolic reprogramming induced by PTEN loss fuels malignant growth and proliferation of prostate cancer cells. Targeted metabolomics analysi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386093/ https://www.ncbi.nlm.nih.gov/pubmed/30854061 http://dx.doi.org/10.3892/ol.2019.9932 |
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author | Zhou, Xin Yang, Xu Sun, Xiang Xu, Xinyuan Li, Xi'an Guo, Yan Wang, Jiancai Li, Xia Yao, Libo Wang, He Shen, Lan |
author_facet | Zhou, Xin Yang, Xu Sun, Xiang Xu, Xinyuan Li, Xi'an Guo, Yan Wang, Jiancai Li, Xia Yao, Libo Wang, He Shen, Lan |
author_sort | Zhou, Xin |
collection | PubMed |
description | The tumor suppressor gene PTEN is one of the most often deleted genes in human prostate cancer. Loss of PTEN is an important event in prostate carcinogenesis. Metabolic reprogramming induced by PTEN loss fuels malignant growth and proliferation of prostate cancer cells. Targeted metabolomics analysis was used to investigate the effects of PTEN loss on intracellular metabolic pathways in prostate cancer cells. DU-145 cells were transfected with PTEN siRNAs (siRNA-1 and siRNA-2) for 48 h, and endogenous PTEN expression was monitored by western blotting. Changes in intracellular metabolites were determined by liquid chromatography-tandem mass chromatography (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS). Most intracellular metabolites involved in glycolysis and glutaminolysis were increased in PTEN knockdown prostate cancer cells. In addition, most intracellular metabolites involved in fatty acid de novo synthesis, fatty acid beta oxidation and branched chain amino acid catabolism were also increased in PTEN knockdown prostate cancer cells. These results revealed that PTEN loss induced the metabolic reprogramming of prostate cancer cells and promoted the malignant proliferation of prostate cancer cells. The present metabolomics analysis indicates that tumor suppressor gene PTEN mutation or deletion can induce metabolic reprogramming in prostate cancer cells and tumorigenesis by altering the metabolic flux of glycolysis, glutaminolysis, fatty acid metabolism and branched chain amino acid catabolism pathways. Metabolic reprogramming is one of the contributors to PTEN-loss driven prostate cancer. |
format | Online Article Text |
id | pubmed-6386093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63860932019-03-08 Effect of PTEN loss on metabolic reprogramming in prostate cancer cells Zhou, Xin Yang, Xu Sun, Xiang Xu, Xinyuan Li, Xi'an Guo, Yan Wang, Jiancai Li, Xia Yao, Libo Wang, He Shen, Lan Oncol Lett Articles The tumor suppressor gene PTEN is one of the most often deleted genes in human prostate cancer. Loss of PTEN is an important event in prostate carcinogenesis. Metabolic reprogramming induced by PTEN loss fuels malignant growth and proliferation of prostate cancer cells. Targeted metabolomics analysis was used to investigate the effects of PTEN loss on intracellular metabolic pathways in prostate cancer cells. DU-145 cells were transfected with PTEN siRNAs (siRNA-1 and siRNA-2) for 48 h, and endogenous PTEN expression was monitored by western blotting. Changes in intracellular metabolites were determined by liquid chromatography-tandem mass chromatography (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS). Most intracellular metabolites involved in glycolysis and glutaminolysis were increased in PTEN knockdown prostate cancer cells. In addition, most intracellular metabolites involved in fatty acid de novo synthesis, fatty acid beta oxidation and branched chain amino acid catabolism were also increased in PTEN knockdown prostate cancer cells. These results revealed that PTEN loss induced the metabolic reprogramming of prostate cancer cells and promoted the malignant proliferation of prostate cancer cells. The present metabolomics analysis indicates that tumor suppressor gene PTEN mutation or deletion can induce metabolic reprogramming in prostate cancer cells and tumorigenesis by altering the metabolic flux of glycolysis, glutaminolysis, fatty acid metabolism and branched chain amino acid catabolism pathways. Metabolic reprogramming is one of the contributors to PTEN-loss driven prostate cancer. D.A. Spandidos 2019-03 2019-01-14 /pmc/articles/PMC6386093/ /pubmed/30854061 http://dx.doi.org/10.3892/ol.2019.9932 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhou, Xin Yang, Xu Sun, Xiang Xu, Xinyuan Li, Xi'an Guo, Yan Wang, Jiancai Li, Xia Yao, Libo Wang, He Shen, Lan Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title | Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title_full | Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title_fullStr | Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title_full_unstemmed | Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title_short | Effect of PTEN loss on metabolic reprogramming in prostate cancer cells |
title_sort | effect of pten loss on metabolic reprogramming in prostate cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386093/ https://www.ncbi.nlm.nih.gov/pubmed/30854061 http://dx.doi.org/10.3892/ol.2019.9932 |
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