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First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases
INTRODUCTION: Aedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance and mechanisms by which the mosquito develops resistance is crucial to minimise disease transmission. AIM: To determine insecti...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386213/ https://www.ncbi.nlm.nih.gov/pubmed/30722810 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.5.1700847 |
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author | Kasai, Shinji Caputo, Beniamino Tsunoda, Takashi Cuong, Tran Chi Maekawa, Yoshihide Lam-Phua, Sai Gek Pichler, Verena Itokawa, Kentaro Murota, Katsunori Komagata, Osamu Yoshida, Chigusa Chung, Han-Hsuan Bellini, Romeo Tsuda, Yoshio Teng, Hwa-Jen Filho, José Luiz de Lima Alves, Luiz Carlos Ng, Lee Ching Minakawa, Noboru Yen, Nguyen Thi Phong, Tran Vu Sawabe, Kyoko Tomita, Takashi |
author_facet | Kasai, Shinji Caputo, Beniamino Tsunoda, Takashi Cuong, Tran Chi Maekawa, Yoshihide Lam-Phua, Sai Gek Pichler, Verena Itokawa, Kentaro Murota, Katsunori Komagata, Osamu Yoshida, Chigusa Chung, Han-Hsuan Bellini, Romeo Tsuda, Yoshio Teng, Hwa-Jen Filho, José Luiz de Lima Alves, Luiz Carlos Ng, Lee Ching Minakawa, Noboru Yen, Nguyen Thi Phong, Tran Vu Sawabe, Kyoko Tomita, Takashi |
author_sort | Kasai, Shinji |
collection | PubMed |
description | INTRODUCTION: Aedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance and mechanisms by which the mosquito develops resistance is crucial to minimise disease transmission. AIM: To determine insecticide resistance status and mechanisms in Ae. albopictus from different geographical regions. METHODS: We sampled 33 populations of Ae. albopictus from Asia, Europe and South America, and tested these for susceptibility to permethrin, a pyrethroid insecticide. In resistant populations, the target site for pyrethroids, a voltage-sensitive sodium channel (Vssc) was genotyped. Three resistant sub-strains, each harbouring a resistance allele homozygously, were established and susceptibilities to three different pyrethroids (with and without a cytochrome P450 inhibitor) were assayed. RESULTS: Most populations of Ae. albopictus tested were highly susceptible to permethrin but a few from Italy and Vietnam (4/33), exhibited high-level resistance. Genotyping studies detected a knockdown resistance (kdr) allele V1016G in Vssc for the first time in Ae. albopictus. Two previously reported kdr alleles, F1534C and F1534S, were also detected. The bioassays indicated that the strain homozygous for the V1016G allele showed much greater levels of pyrethroid resistance than other strains harbouring F1534C or F1534S. CONCLUSION: The V1016G allele was detected in bothAsian and Italian Ae. albopictus populations, thus a spread of this allele beyond Italy in Europe cannot be ruled out. This study emphasises the necessity to frequently and regularly monitor the V1016G allele in Ae. albopictus, particularly where this mosquito species is the main vector of arboviruses. |
format | Online Article Text |
id | pubmed-6386213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | European Centre for Disease Prevention and Control (ECDC) |
record_format | MEDLINE/PubMed |
spelling | pubmed-63862132019-03-01 First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases Kasai, Shinji Caputo, Beniamino Tsunoda, Takashi Cuong, Tran Chi Maekawa, Yoshihide Lam-Phua, Sai Gek Pichler, Verena Itokawa, Kentaro Murota, Katsunori Komagata, Osamu Yoshida, Chigusa Chung, Han-Hsuan Bellini, Romeo Tsuda, Yoshio Teng, Hwa-Jen Filho, José Luiz de Lima Alves, Luiz Carlos Ng, Lee Ching Minakawa, Noboru Yen, Nguyen Thi Phong, Tran Vu Sawabe, Kyoko Tomita, Takashi Euro Surveill Research INTRODUCTION: Aedes albopictus (Skuse) is an important vector of arboviral diseases, including dengue, chikungunya and Zika virus disease. Monitoring insecticide resistance and mechanisms by which the mosquito develops resistance is crucial to minimise disease transmission. AIM: To determine insecticide resistance status and mechanisms in Ae. albopictus from different geographical regions. METHODS: We sampled 33 populations of Ae. albopictus from Asia, Europe and South America, and tested these for susceptibility to permethrin, a pyrethroid insecticide. In resistant populations, the target site for pyrethroids, a voltage-sensitive sodium channel (Vssc) was genotyped. Three resistant sub-strains, each harbouring a resistance allele homozygously, were established and susceptibilities to three different pyrethroids (with and without a cytochrome P450 inhibitor) were assayed. RESULTS: Most populations of Ae. albopictus tested were highly susceptible to permethrin but a few from Italy and Vietnam (4/33), exhibited high-level resistance. Genotyping studies detected a knockdown resistance (kdr) allele V1016G in Vssc for the first time in Ae. albopictus. Two previously reported kdr alleles, F1534C and F1534S, were also detected. The bioassays indicated that the strain homozygous for the V1016G allele showed much greater levels of pyrethroid resistance than other strains harbouring F1534C or F1534S. CONCLUSION: The V1016G allele was detected in bothAsian and Italian Ae. albopictus populations, thus a spread of this allele beyond Italy in Europe cannot be ruled out. This study emphasises the necessity to frequently and regularly monitor the V1016G allele in Ae. albopictus, particularly where this mosquito species is the main vector of arboviruses. European Centre for Disease Prevention and Control (ECDC) 2019-01-31 /pmc/articles/PMC6386213/ /pubmed/30722810 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.5.1700847 Text en This article is copyright of the authors or their affiliated institutions, 2019. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
spellingShingle | Research Kasai, Shinji Caputo, Beniamino Tsunoda, Takashi Cuong, Tran Chi Maekawa, Yoshihide Lam-Phua, Sai Gek Pichler, Verena Itokawa, Kentaro Murota, Katsunori Komagata, Osamu Yoshida, Chigusa Chung, Han-Hsuan Bellini, Romeo Tsuda, Yoshio Teng, Hwa-Jen Filho, José Luiz de Lima Alves, Luiz Carlos Ng, Lee Ching Minakawa, Noboru Yen, Nguyen Thi Phong, Tran Vu Sawabe, Kyoko Tomita, Takashi First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title | First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title_full | First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title_fullStr | First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title_full_unstemmed | First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title_short | First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases |
title_sort | first detection of a vssc allele v1016g conferring a high level of insecticide resistance in aedes albopictus collected from europe (italy) and asia (vietnam), 2016: a new emerging threat to controlling arboviral diseases |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386213/ https://www.ncbi.nlm.nih.gov/pubmed/30722810 http://dx.doi.org/10.2807/1560-7917.ES.2019.24.5.1700847 |
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