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Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos

Plant tissue architecture and organ morphogenesis rely on the proper orientation of cell divisions. Previous attempts to predict division planes from cell geometry in plants mostly focused on 2D symmetric divisions. Using the stereotyped division patterns of Arabidopsis thaliana early embryogenesis,...

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Autores principales: Moukhtar, Julien, Trubuil, Alain, Belcram, Katia, Legland, David, Khadir, Zhor, Urbain, Aurélie, Palauqui, Jean-Christophe, Andrey, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386405/
https://www.ncbi.nlm.nih.gov/pubmed/30742612
http://dx.doi.org/10.1371/journal.pcbi.1006771
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author Moukhtar, Julien
Trubuil, Alain
Belcram, Katia
Legland, David
Khadir, Zhor
Urbain, Aurélie
Palauqui, Jean-Christophe
Andrey, Philippe
author_facet Moukhtar, Julien
Trubuil, Alain
Belcram, Katia
Legland, David
Khadir, Zhor
Urbain, Aurélie
Palauqui, Jean-Christophe
Andrey, Philippe
author_sort Moukhtar, Julien
collection PubMed
description Plant tissue architecture and organ morphogenesis rely on the proper orientation of cell divisions. Previous attempts to predict division planes from cell geometry in plants mostly focused on 2D symmetric divisions. Using the stereotyped division patterns of Arabidopsis thaliana early embryogenesis, we investigated geometrical principles underlying plane selection in symmetric and in asymmetric divisions within complex 3D cell shapes. Introducing a 3D computational model of cell division, we show that area minimization constrained on passing through the cell centroid predicts observed divisions. Our results suggest that the positioning of division planes ensues from cell geometry and gives rise to spatially organized cell types with stereotyped shapes, thus underlining the role of self-organization in the developing architecture of the embryo. Our data further suggested the rule could be interpreted as surface minimization constrained by the nucleus position, which was validated using live imaging of cell divisions in the stomatal cell lineage.
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spelling pubmed-63864052019-03-08 Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos Moukhtar, Julien Trubuil, Alain Belcram, Katia Legland, David Khadir, Zhor Urbain, Aurélie Palauqui, Jean-Christophe Andrey, Philippe PLoS Comput Biol Research Article Plant tissue architecture and organ morphogenesis rely on the proper orientation of cell divisions. Previous attempts to predict division planes from cell geometry in plants mostly focused on 2D symmetric divisions. Using the stereotyped division patterns of Arabidopsis thaliana early embryogenesis, we investigated geometrical principles underlying plane selection in symmetric and in asymmetric divisions within complex 3D cell shapes. Introducing a 3D computational model of cell division, we show that area minimization constrained on passing through the cell centroid predicts observed divisions. Our results suggest that the positioning of division planes ensues from cell geometry and gives rise to spatially organized cell types with stereotyped shapes, thus underlining the role of self-organization in the developing architecture of the embryo. Our data further suggested the rule could be interpreted as surface minimization constrained by the nucleus position, which was validated using live imaging of cell divisions in the stomatal cell lineage. Public Library of Science 2019-02-11 /pmc/articles/PMC6386405/ /pubmed/30742612 http://dx.doi.org/10.1371/journal.pcbi.1006771 Text en © 2019 Moukhtar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Moukhtar, Julien
Trubuil, Alain
Belcram, Katia
Legland, David
Khadir, Zhor
Urbain, Aurélie
Palauqui, Jean-Christophe
Andrey, Philippe
Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title_full Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title_fullStr Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title_full_unstemmed Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title_short Cell geometry determines symmetric and asymmetric division plane selection in Arabidopsis early embryos
title_sort cell geometry determines symmetric and asymmetric division plane selection in arabidopsis early embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386405/
https://www.ncbi.nlm.nih.gov/pubmed/30742612
http://dx.doi.org/10.1371/journal.pcbi.1006771
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