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Extraction-free whole transcriptome gene expression analysis of FFPE sections and histology-directed subareas of tissue

We describe the use of a ligation-based targeted whole transcriptome expression profiling assay, TempO-Seq, to profile formalin-fixed paraffin-embedded (FFPE) tissue, including H&E stained FFPE tissue, by directly lysing tissue scraped from slides without extracting RNA or converting the RNA to...

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Detalles Bibliográficos
Autores principales:	Trejo, Christy L., Babić, Miloš, Imler, Elliot, Gonzalez, Migdalia, Bibikov, Sergei I., Shepard, Peter J., VanSteenhouse, Harper C., Yeakley, Joanne M., Seligmann, Bruce E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386473/ https://www.ncbi.nlm.nih.gov/pubmed/30794557 http://dx.doi.org/10.1371/journal.pone.0212031

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