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Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015

Cassava mosaic disease, one of the ten most economically important crop viral diseases in the world, was first reported in Southeast Asia from a single plantation in Cambodia in 2015. To determine the presence and incidence of Sri Lankan cassava mosaic virus (SLCMV) one year after first detection, a...

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Autores principales: Minato, Nami, Sok, Sophearith, Chen, Songbi, Delaquis, Erik, Phirun, Iv, Le, Vi Xuan, Burra, Dharani D., Newby, Jonathan C., Wyckhuys, Kris A. G., de Haan, Stef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386488/
https://www.ncbi.nlm.nih.gov/pubmed/30794679
http://dx.doi.org/10.1371/journal.pone.0212780
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author Minato, Nami
Sok, Sophearith
Chen, Songbi
Delaquis, Erik
Phirun, Iv
Le, Vi Xuan
Burra, Dharani D.
Newby, Jonathan C.
Wyckhuys, Kris A. G.
de Haan, Stef
author_facet Minato, Nami
Sok, Sophearith
Chen, Songbi
Delaquis, Erik
Phirun, Iv
Le, Vi Xuan
Burra, Dharani D.
Newby, Jonathan C.
Wyckhuys, Kris A. G.
de Haan, Stef
author_sort Minato, Nami
collection PubMed
description Cassava mosaic disease, one of the ten most economically important crop viral diseases in the world, was first reported in Southeast Asia from a single plantation in Cambodia in 2015. To determine the presence and incidence of Sri Lankan cassava mosaic virus (SLCMV) one year after first detection, a total of 6,480 samples from 419 fields were systematically collected from cassava production areas across Cambodia (3,840 samples; 240 fields) and Vietnam (2,640samples; 179 fields) in the 2016 cropping season. Using PCR-based diagnostics, we identified 49 SLCMV-infected plants from nine fields, representing 2% of the total number of fields sampled. Infected fields were geographically restricted to two provinces of Eastern Cambodia, while no infection was detected from any of the other sampled sites in either country. Symptom expression patterns in infected plants suggested that SLCMV may have been transmitted both through infected planting materials, and by Bemisia tabaci, the known whitefly vector of SLCMV. In addition, 14% of virus infected plants did not express typical symptoms of cassava mosaic disease on their leaves, highlighting that molecular-based validation is needed to confirm the presence of SLCMV in the field. None of the owners of the SLCMV-infected fields indicated acquired planting materials from the plantation in Ratanakiri where SLCMV was first reported. The surveillance baseline data generated for both countries is discussed in light of future options to control and manage cassava mosaic disease.
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spelling pubmed-63864882019-03-09 Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015 Minato, Nami Sok, Sophearith Chen, Songbi Delaquis, Erik Phirun, Iv Le, Vi Xuan Burra, Dharani D. Newby, Jonathan C. Wyckhuys, Kris A. G. de Haan, Stef PLoS One Research Article Cassava mosaic disease, one of the ten most economically important crop viral diseases in the world, was first reported in Southeast Asia from a single plantation in Cambodia in 2015. To determine the presence and incidence of Sri Lankan cassava mosaic virus (SLCMV) one year after first detection, a total of 6,480 samples from 419 fields were systematically collected from cassava production areas across Cambodia (3,840 samples; 240 fields) and Vietnam (2,640samples; 179 fields) in the 2016 cropping season. Using PCR-based diagnostics, we identified 49 SLCMV-infected plants from nine fields, representing 2% of the total number of fields sampled. Infected fields were geographically restricted to two provinces of Eastern Cambodia, while no infection was detected from any of the other sampled sites in either country. Symptom expression patterns in infected plants suggested that SLCMV may have been transmitted both through infected planting materials, and by Bemisia tabaci, the known whitefly vector of SLCMV. In addition, 14% of virus infected plants did not express typical symptoms of cassava mosaic disease on their leaves, highlighting that molecular-based validation is needed to confirm the presence of SLCMV in the field. None of the owners of the SLCMV-infected fields indicated acquired planting materials from the plantation in Ratanakiri where SLCMV was first reported. The surveillance baseline data generated for both countries is discussed in light of future options to control and manage cassava mosaic disease. Public Library of Science 2019-02-22 /pmc/articles/PMC6386488/ /pubmed/30794679 http://dx.doi.org/10.1371/journal.pone.0212780 Text en © 2019 Minato et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Minato, Nami
Sok, Sophearith
Chen, Songbi
Delaquis, Erik
Phirun, Iv
Le, Vi Xuan
Burra, Dharani D.
Newby, Jonathan C.
Wyckhuys, Kris A. G.
de Haan, Stef
Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title_full Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title_fullStr Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title_full_unstemmed Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title_short Surveillance for Sri Lankan cassava mosaic virus (SLCMV) in Cambodia and Vietnam one year after its initial detection in a single plantation in 2015
title_sort surveillance for sri lankan cassava mosaic virus (slcmv) in cambodia and vietnam one year after its initial detection in a single plantation in 2015
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386488/
https://www.ncbi.nlm.nih.gov/pubmed/30794679
http://dx.doi.org/10.1371/journal.pone.0212780
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