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NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease
The role of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) has been highlighted in mechanisms underlying inflammatory and neuropathic pain processes. The present study was designed to investigate whether NF-κB signaling is associated with pain-related neuropeptide expression...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386867/ https://www.ncbi.nlm.nih.gov/pubmed/30717434 http://dx.doi.org/10.3390/ijms20030658 |
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author | Ahmed, Aisha S. Berg, Svante Alkass, Kanar Druid, Henrik Hart, David A. Svensson, Camilla I. Kosek, Eva |
author_facet | Ahmed, Aisha S. Berg, Svante Alkass, Kanar Druid, Henrik Hart, David A. Svensson, Camilla I. Kosek, Eva |
author_sort | Ahmed, Aisha S. |
collection | PubMed |
description | The role of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) has been highlighted in mechanisms underlying inflammatory and neuropathic pain processes. The present study was designed to investigate whether NF-κB signaling is associated with pain-related neuropeptide expression in patients with chronic back pain related to degenerative disc disease (DDD). Intervertebral disc (IVD) tissues were collected from forty DDD patients undergoing disc replacement or fusion surgery, and from eighteen postmortem (PM) control subjects. RELA, NFKB1, CGRP, TAC1, TRPV1, and MMP-3 gene expression were analyzed by RT-qPCR, while NF-κB subunit RelA and NF-κB1–DNA binding in nuclear extracts and calcitonin gene related peptide (CGRP), substance P (SP), and transient receptor potential, subfamily V, member 1 (TRPV1) protein levels in cytosolic extracts of tissues were assessed by enzyme-linked immunosorbent assay (ELISA). An upregulated NF-κB1–DNA binding, and higher CGRP and TRPV1 protein levels were observed in DDD patients compared to PM controls. In DDD patients, NF-κB1–DNA binding was positively correlated with nuclear RelA levels. Moreover, NF-κB1–DNA binding was positively associated with TRPV1 and MMP-3 gene and SP and TRPV1 protein expression in DDD patients. Our results indicate that the expression of SP and TRPV1 in IVD tissues was associated with NF-κB activation. Moreover, NF-κB may be involved in the generation or maintenance of peripheral pain mechanisms by the regulation of pain-related neuropeptide expression in DDD patients. |
format | Online Article Text |
id | pubmed-6386867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63868672019-02-27 NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease Ahmed, Aisha S. Berg, Svante Alkass, Kanar Druid, Henrik Hart, David A. Svensson, Camilla I. Kosek, Eva Int J Mol Sci Article The role of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) has been highlighted in mechanisms underlying inflammatory and neuropathic pain processes. The present study was designed to investigate whether NF-κB signaling is associated with pain-related neuropeptide expression in patients with chronic back pain related to degenerative disc disease (DDD). Intervertebral disc (IVD) tissues were collected from forty DDD patients undergoing disc replacement or fusion surgery, and from eighteen postmortem (PM) control subjects. RELA, NFKB1, CGRP, TAC1, TRPV1, and MMP-3 gene expression were analyzed by RT-qPCR, while NF-κB subunit RelA and NF-κB1–DNA binding in nuclear extracts and calcitonin gene related peptide (CGRP), substance P (SP), and transient receptor potential, subfamily V, member 1 (TRPV1) protein levels in cytosolic extracts of tissues were assessed by enzyme-linked immunosorbent assay (ELISA). An upregulated NF-κB1–DNA binding, and higher CGRP and TRPV1 protein levels were observed in DDD patients compared to PM controls. In DDD patients, NF-κB1–DNA binding was positively correlated with nuclear RelA levels. Moreover, NF-κB1–DNA binding was positively associated with TRPV1 and MMP-3 gene and SP and TRPV1 protein expression in DDD patients. Our results indicate that the expression of SP and TRPV1 in IVD tissues was associated with NF-κB activation. Moreover, NF-κB may be involved in the generation or maintenance of peripheral pain mechanisms by the regulation of pain-related neuropeptide expression in DDD patients. MDPI 2019-02-03 /pmc/articles/PMC6386867/ /pubmed/30717434 http://dx.doi.org/10.3390/ijms20030658 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ahmed, Aisha S. Berg, Svante Alkass, Kanar Druid, Henrik Hart, David A. Svensson, Camilla I. Kosek, Eva NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title | NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title_full | NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title_fullStr | NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title_full_unstemmed | NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title_short | NF-κB-Associated Pain-Related Neuropeptide Expression in Patients with Degenerative Disc Disease |
title_sort | nf-κb-associated pain-related neuropeptide expression in patients with degenerative disc disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386867/ https://www.ncbi.nlm.nih.gov/pubmed/30717434 http://dx.doi.org/10.3390/ijms20030658 |
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