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Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387113/ https://www.ncbi.nlm.nih.gov/pubmed/30696003 http://dx.doi.org/10.3390/s19030534 |
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author | Zhang, Mingkun Yang, Zhongbo Tang, Mingjie Wang, Deqiang Wang, Huabin Yan, Shihan Wei, Dongshan Cui, Hong-Liang |
author_facet | Zhang, Mingkun Yang, Zhongbo Tang, Mingjie Wang, Deqiang Wang, Huabin Yan, Shihan Wei, Dongshan Cui, Hong-Liang |
author_sort | Zhang, Mingkun |
collection | PubMed |
description | Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatures of microcystin aptamer (a linear single-stranded DNA with 60 nucleotides) dissolved in TE buffer with different concentrations. The microfluidic chip made of silicon includes thousands of 2.4 μm × 2.4 μm square-cross-section channels. One repeatable terahertz absorption signature is detected and recognized around 830 GHz, fitted to a Lorentz oscillator. This signature is theorized to originate from the bending of hydrogen bonds formed between adjacent hydrated DNA bases surrounded by water molecules. Furthermore, the low-lying vibrational modes are also investigated by molecular dynamics simulations which suggest that strong resonant oscillations are highly probable in the 815–830 GHz frequency band. |
format | Online Article Text |
id | pubmed-6387113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63871132019-02-26 Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip Zhang, Mingkun Yang, Zhongbo Tang, Mingjie Wang, Deqiang Wang, Huabin Yan, Shihan Wei, Dongshan Cui, Hong-Liang Sensors (Basel) Article Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatures of microcystin aptamer (a linear single-stranded DNA with 60 nucleotides) dissolved in TE buffer with different concentrations. The microfluidic chip made of silicon includes thousands of 2.4 μm × 2.4 μm square-cross-section channels. One repeatable terahertz absorption signature is detected and recognized around 830 GHz, fitted to a Lorentz oscillator. This signature is theorized to originate from the bending of hydrogen bonds formed between adjacent hydrated DNA bases surrounded by water molecules. Furthermore, the low-lying vibrational modes are also investigated by molecular dynamics simulations which suggest that strong resonant oscillations are highly probable in the 815–830 GHz frequency band. MDPI 2019-01-28 /pmc/articles/PMC6387113/ /pubmed/30696003 http://dx.doi.org/10.3390/s19030534 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Mingkun Yang, Zhongbo Tang, Mingjie Wang, Deqiang Wang, Huabin Yan, Shihan Wei, Dongshan Cui, Hong-Liang Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title | Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title_full | Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title_fullStr | Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title_full_unstemmed | Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title_short | Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip |
title_sort | terahertz spectroscopic signatures of microcystin aptamer solution probed with a microfluidic chip |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387113/ https://www.ncbi.nlm.nih.gov/pubmed/30696003 http://dx.doi.org/10.3390/s19030534 |
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