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Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip

Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatu...

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Detalles Bibliográficos
Autores principales: Zhang, Mingkun, Yang, Zhongbo, Tang, Mingjie, Wang, Deqiang, Wang, Huabin, Yan, Shihan, Wei, Dongshan, Cui, Hong-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387113/
https://www.ncbi.nlm.nih.gov/pubmed/30696003
http://dx.doi.org/10.3390/s19030534
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author Zhang, Mingkun
Yang, Zhongbo
Tang, Mingjie
Wang, Deqiang
Wang, Huabin
Yan, Shihan
Wei, Dongshan
Cui, Hong-Liang
author_facet Zhang, Mingkun
Yang, Zhongbo
Tang, Mingjie
Wang, Deqiang
Wang, Huabin
Yan, Shihan
Wei, Dongshan
Cui, Hong-Liang
author_sort Zhang, Mingkun
collection PubMed
description Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatures of microcystin aptamer (a linear single-stranded DNA with 60 nucleotides) dissolved in TE buffer with different concentrations. The microfluidic chip made of silicon includes thousands of 2.4 μm × 2.4 μm square-cross-section channels. One repeatable terahertz absorption signature is detected and recognized around 830 GHz, fitted to a Lorentz oscillator. This signature is theorized to originate from the bending of hydrogen bonds formed between adjacent hydrated DNA bases surrounded by water molecules. Furthermore, the low-lying vibrational modes are also investigated by molecular dynamics simulations which suggest that strong resonant oscillations are highly probable in the 815–830 GHz frequency band.
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spelling pubmed-63871132019-02-26 Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip Zhang, Mingkun Yang, Zhongbo Tang, Mingjie Wang, Deqiang Wang, Huabin Yan, Shihan Wei, Dongshan Cui, Hong-Liang Sensors (Basel) Article Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatures of microcystin aptamer (a linear single-stranded DNA with 60 nucleotides) dissolved in TE buffer with different concentrations. The microfluidic chip made of silicon includes thousands of 2.4 μm × 2.4 μm square-cross-section channels. One repeatable terahertz absorption signature is detected and recognized around 830 GHz, fitted to a Lorentz oscillator. This signature is theorized to originate from the bending of hydrogen bonds formed between adjacent hydrated DNA bases surrounded by water molecules. Furthermore, the low-lying vibrational modes are also investigated by molecular dynamics simulations which suggest that strong resonant oscillations are highly probable in the 815–830 GHz frequency band. MDPI 2019-01-28 /pmc/articles/PMC6387113/ /pubmed/30696003 http://dx.doi.org/10.3390/s19030534 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Mingkun
Yang, Zhongbo
Tang, Mingjie
Wang, Deqiang
Wang, Huabin
Yan, Shihan
Wei, Dongshan
Cui, Hong-Liang
Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title_full Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title_fullStr Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title_full_unstemmed Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title_short Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip
title_sort terahertz spectroscopic signatures of microcystin aptamer solution probed with a microfluidic chip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387113/
https://www.ncbi.nlm.nih.gov/pubmed/30696003
http://dx.doi.org/10.3390/s19030534
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