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Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk

The rapid analysis of stilbene estrogens is crucially important in the environment, food and health sectors, but quantitation of lower detection limit for stilbene estrogens persists as a severe challenge. We herein described a homologous and sensitive fluorescence polarization (FP) assay based on e...

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Autores principales: Guan, Tianzhu, Sun, Yonghai, Wang, Yongjun, Li, Zhuolin, Li, Tiezhu, Hou, Ligang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387142/
https://www.ncbi.nlm.nih.gov/pubmed/30744198
http://dx.doi.org/10.3390/ijms20030744
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author Guan, Tianzhu
Sun, Yonghai
Wang, Yongjun
Li, Zhuolin
Li, Tiezhu
Hou, Ligang
author_facet Guan, Tianzhu
Sun, Yonghai
Wang, Yongjun
Li, Zhuolin
Li, Tiezhu
Hou, Ligang
author_sort Guan, Tianzhu
collection PubMed
description The rapid analysis of stilbene estrogens is crucially important in the environment, food and health sectors, but quantitation of lower detection limit for stilbene estrogens persists as a severe challenge. We herein described a homologous and sensitive fluorescence polarization (FP) assay based on estrogen receptor α ligand binding domain (ER-LBD) to monitor stilbene estrogens in milk. Under optimal conditions, the half maximal inhibitory concentrations (IC(50)) of the FP assay were 9.27 nM, 12.94 nM, and 22.38 nM for hexestrol, dienestrol and diethylstilbestrol, respectively. And the corresponding limits of detection (LOD) values were 2.94 nM, 2.89 nM, and 3.12 nM. Finally, the assay was applied to determine the stilbenes in milk samples where the mean recoveries ranged from 95.76% to 112.78% and the coefficients of variation (CV) below 12.00%. Furtherly, we have focused our study on high cross-reactivity phenomena by using two in silico approaches, including molecular docking analysis and topology analysis. Overall, docking results show that several residues in the hydrophobic pocket produce hydrophobic interactions with the tested drug molecules, which contribute to the stability of their binding. In this paper, we conclude that the FP method is suitable for the rapid detection of stilbenes in milk samples, requiring no expensive analytical equipment or time-consuming sample preparation. This work offers a practical approach that applies bioscience technology in food safety testing and improves analytical speed and laboratory efficiency.
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spelling pubmed-63871422019-02-27 Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk Guan, Tianzhu Sun, Yonghai Wang, Yongjun Li, Zhuolin Li, Tiezhu Hou, Ligang Int J Mol Sci Article The rapid analysis of stilbene estrogens is crucially important in the environment, food and health sectors, but quantitation of lower detection limit for stilbene estrogens persists as a severe challenge. We herein described a homologous and sensitive fluorescence polarization (FP) assay based on estrogen receptor α ligand binding domain (ER-LBD) to monitor stilbene estrogens in milk. Under optimal conditions, the half maximal inhibitory concentrations (IC(50)) of the FP assay were 9.27 nM, 12.94 nM, and 22.38 nM for hexestrol, dienestrol and diethylstilbestrol, respectively. And the corresponding limits of detection (LOD) values were 2.94 nM, 2.89 nM, and 3.12 nM. Finally, the assay was applied to determine the stilbenes in milk samples where the mean recoveries ranged from 95.76% to 112.78% and the coefficients of variation (CV) below 12.00%. Furtherly, we have focused our study on high cross-reactivity phenomena by using two in silico approaches, including molecular docking analysis and topology analysis. Overall, docking results show that several residues in the hydrophobic pocket produce hydrophobic interactions with the tested drug molecules, which contribute to the stability of their binding. In this paper, we conclude that the FP method is suitable for the rapid detection of stilbenes in milk samples, requiring no expensive analytical equipment or time-consuming sample preparation. This work offers a practical approach that applies bioscience technology in food safety testing and improves analytical speed and laboratory efficiency. MDPI 2019-02-10 /pmc/articles/PMC6387142/ /pubmed/30744198 http://dx.doi.org/10.3390/ijms20030744 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guan, Tianzhu
Sun, Yonghai
Wang, Yongjun
Li, Zhuolin
Li, Tiezhu
Hou, Ligang
Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title_full Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title_fullStr Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title_full_unstemmed Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title_short Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
title_sort multi-residue method for the analysis of stilbene estrogens in milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387142/
https://www.ncbi.nlm.nih.gov/pubmed/30744198
http://dx.doi.org/10.3390/ijms20030744
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