Cargando…

Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires

Pregnancy is a state where high and stage-dependent plasticity of the maternal immune system is necessary in order to equilibrate between immunosuppression of harmful responses towards the fetus and ability to fight infections. TCR γδ cells have been implicated in the responses in infectious disease...

Descripción completa

Detalles Bibliográficos
Autores principales: Terzieva, Antonia, Dimitrova, Violeta, Djerov, Lyubomir, Dimitrova, Petya, Zapryanova, Silvina, Hristova, Iana, Vangelov, Ivaylo, Dimova, Tanya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387174/
https://www.ncbi.nlm.nih.gov/pubmed/30764544
http://dx.doi.org/10.3390/ijms20030687
_version_ 1783397512796700672
author Terzieva, Antonia
Dimitrova, Violeta
Djerov, Lyubomir
Dimitrova, Petya
Zapryanova, Silvina
Hristova, Iana
Vangelov, Ivaylo
Dimova, Tanya
author_facet Terzieva, Antonia
Dimitrova, Violeta
Djerov, Lyubomir
Dimitrova, Petya
Zapryanova, Silvina
Hristova, Iana
Vangelov, Ivaylo
Dimova, Tanya
author_sort Terzieva, Antonia
collection PubMed
description Pregnancy is a state where high and stage-dependent plasticity of the maternal immune system is necessary in order to equilibrate between immunosuppression of harmful responses towards the fetus and ability to fight infections. TCR γδ cells have been implicated in the responses in infectious diseases, in the regulation of immune responses, and in tissue homeostasis and repair. The variety of functions makes γδ T cells a particularly interesting population during pregnancy. In this study, we investigated the proportion, phenotype and TCR γ and δ repertoires of γδ T cells at the maternal–fetal interface and in the blood of pregnant women using FACS, immunohistochemistry and spectratyping. We found an enrichment of activated and terminally differentiated pro-inflammatory γδ T-cell effectors with specific location in the human decidua during early pregnancy, while no significant changes in their counterparts in the blood of pregnant women were observed. Our spectratyping data revealed polyclonal CDR3 repertoires of the δ1, δ2 and δ3 chains and γ2, γ3, γ4 and γ5 chains and oligoclonal and highly restricted CDR3γ9 repertoire of γδ T cells in the decidua and blood of pregnant women. Early pregnancy induces recruitment of differentiated pro-inflammatory γδ T-cell effectors with diverse TCR repertoires at the maternal–fetal interface.
format Online
Article
Text
id pubmed-6387174
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63871742019-02-27 Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires Terzieva, Antonia Dimitrova, Violeta Djerov, Lyubomir Dimitrova, Petya Zapryanova, Silvina Hristova, Iana Vangelov, Ivaylo Dimova, Tanya Int J Mol Sci Article Pregnancy is a state where high and stage-dependent plasticity of the maternal immune system is necessary in order to equilibrate between immunosuppression of harmful responses towards the fetus and ability to fight infections. TCR γδ cells have been implicated in the responses in infectious diseases, in the regulation of immune responses, and in tissue homeostasis and repair. The variety of functions makes γδ T cells a particularly interesting population during pregnancy. In this study, we investigated the proportion, phenotype and TCR γ and δ repertoires of γδ T cells at the maternal–fetal interface and in the blood of pregnant women using FACS, immunohistochemistry and spectratyping. We found an enrichment of activated and terminally differentiated pro-inflammatory γδ T-cell effectors with specific location in the human decidua during early pregnancy, while no significant changes in their counterparts in the blood of pregnant women were observed. Our spectratyping data revealed polyclonal CDR3 repertoires of the δ1, δ2 and δ3 chains and γ2, γ3, γ4 and γ5 chains and oligoclonal and highly restricted CDR3γ9 repertoire of γδ T cells in the decidua and blood of pregnant women. Early pregnancy induces recruitment of differentiated pro-inflammatory γδ T-cell effectors with diverse TCR repertoires at the maternal–fetal interface. MDPI 2019-02-05 /pmc/articles/PMC6387174/ /pubmed/30764544 http://dx.doi.org/10.3390/ijms20030687 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Terzieva, Antonia
Dimitrova, Violeta
Djerov, Lyubomir
Dimitrova, Petya
Zapryanova, Silvina
Hristova, Iana
Vangelov, Ivaylo
Dimova, Tanya
Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title_full Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title_fullStr Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title_full_unstemmed Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title_short Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
title_sort early pregnancy human decidua is enriched with activated, fully differentiated and pro-inflammatory gamma/delta t cells with diverse tcr repertoires
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387174/
https://www.ncbi.nlm.nih.gov/pubmed/30764544
http://dx.doi.org/10.3390/ijms20030687
work_keys_str_mv AT terzievaantonia earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT dimitrovavioleta earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT djerovlyubomir earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT dimitrovapetya earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT zapryanovasilvina earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT hristovaiana earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT vangelovivaylo earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires
AT dimovatanya earlypregnancyhumandeciduaisenrichedwithactivatedfullydifferentiatedandproinflammatorygammadeltatcellswithdiversetcrrepertoires