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α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the prese...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387298/ https://www.ncbi.nlm.nih.gov/pubmed/30708965 http://dx.doi.org/10.3390/ijms20030609 |
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author | Camiolo, Giuseppina Tibullo, Daniele Giallongo, Cesarina Romano, Alessandra Parrinello, Nunziatina L. Musumeci, Giuseppe Di Rosa, Michelino Vicario, Nunzio Brundo, Maria V. Amenta, Francesco Ferrante, Margherita Copat, Chiara Avola, Roberto Li Volti, Giovanni Salvaggio, Antonio Di Raimondo, Francesco Palumbo, Giuseppe A. |
author_facet | Camiolo, Giuseppina Tibullo, Daniele Giallongo, Cesarina Romano, Alessandra Parrinello, Nunziatina L. Musumeci, Giuseppe Di Rosa, Michelino Vicario, Nunzio Brundo, Maria V. Amenta, Francesco Ferrante, Margherita Copat, Chiara Avola, Roberto Li Volti, Giovanni Salvaggio, Antonio Di Raimondo, Francesco Palumbo, Giuseppe A. |
author_sort | Camiolo, Giuseppina |
collection | PubMed |
description | Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system. |
format | Online Article Text |
id | pubmed-6387298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63872982019-02-27 α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload Camiolo, Giuseppina Tibullo, Daniele Giallongo, Cesarina Romano, Alessandra Parrinello, Nunziatina L. Musumeci, Giuseppe Di Rosa, Michelino Vicario, Nunzio Brundo, Maria V. Amenta, Francesco Ferrante, Margherita Copat, Chiara Avola, Roberto Li Volti, Giovanni Salvaggio, Antonio Di Raimondo, Francesco Palumbo, Giuseppe A. Int J Mol Sci Article Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system. MDPI 2019-01-31 /pmc/articles/PMC6387298/ /pubmed/30708965 http://dx.doi.org/10.3390/ijms20030609 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Camiolo, Giuseppina Tibullo, Daniele Giallongo, Cesarina Romano, Alessandra Parrinello, Nunziatina L. Musumeci, Giuseppe Di Rosa, Michelino Vicario, Nunzio Brundo, Maria V. Amenta, Francesco Ferrante, Margherita Copat, Chiara Avola, Roberto Li Volti, Giovanni Salvaggio, Antonio Di Raimondo, Francesco Palumbo, Giuseppe A. α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title | α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title_full | α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title_fullStr | α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title_full_unstemmed | α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title_short | α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload |
title_sort | α-lipoic acid reduces iron-induced toxicity and oxidative stress in a model of iron overload |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387298/ https://www.ncbi.nlm.nih.gov/pubmed/30708965 http://dx.doi.org/10.3390/ijms20030609 |
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