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α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload

Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the prese...

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Autores principales: Camiolo, Giuseppina, Tibullo, Daniele, Giallongo, Cesarina, Romano, Alessandra, Parrinello, Nunziatina L., Musumeci, Giuseppe, Di Rosa, Michelino, Vicario, Nunzio, Brundo, Maria V., Amenta, Francesco, Ferrante, Margherita, Copat, Chiara, Avola, Roberto, Li Volti, Giovanni, Salvaggio, Antonio, Di Raimondo, Francesco, Palumbo, Giuseppe A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387298/
https://www.ncbi.nlm.nih.gov/pubmed/30708965
http://dx.doi.org/10.3390/ijms20030609
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author Camiolo, Giuseppina
Tibullo, Daniele
Giallongo, Cesarina
Romano, Alessandra
Parrinello, Nunziatina L.
Musumeci, Giuseppe
Di Rosa, Michelino
Vicario, Nunzio
Brundo, Maria V.
Amenta, Francesco
Ferrante, Margherita
Copat, Chiara
Avola, Roberto
Li Volti, Giovanni
Salvaggio, Antonio
Di Raimondo, Francesco
Palumbo, Giuseppe A.
author_facet Camiolo, Giuseppina
Tibullo, Daniele
Giallongo, Cesarina
Romano, Alessandra
Parrinello, Nunziatina L.
Musumeci, Giuseppe
Di Rosa, Michelino
Vicario, Nunzio
Brundo, Maria V.
Amenta, Francesco
Ferrante, Margherita
Copat, Chiara
Avola, Roberto
Li Volti, Giovanni
Salvaggio, Antonio
Di Raimondo, Francesco
Palumbo, Giuseppe A.
author_sort Camiolo, Giuseppina
collection PubMed
description Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system.
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spelling pubmed-63872982019-02-27 α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload Camiolo, Giuseppina Tibullo, Daniele Giallongo, Cesarina Romano, Alessandra Parrinello, Nunziatina L. Musumeci, Giuseppe Di Rosa, Michelino Vicario, Nunzio Brundo, Maria V. Amenta, Francesco Ferrante, Margherita Copat, Chiara Avola, Roberto Li Volti, Giovanni Salvaggio, Antonio Di Raimondo, Francesco Palumbo, Giuseppe A. Int J Mol Sci Article Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system. MDPI 2019-01-31 /pmc/articles/PMC6387298/ /pubmed/30708965 http://dx.doi.org/10.3390/ijms20030609 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camiolo, Giuseppina
Tibullo, Daniele
Giallongo, Cesarina
Romano, Alessandra
Parrinello, Nunziatina L.
Musumeci, Giuseppe
Di Rosa, Michelino
Vicario, Nunzio
Brundo, Maria V.
Amenta, Francesco
Ferrante, Margherita
Copat, Chiara
Avola, Roberto
Li Volti, Giovanni
Salvaggio, Antonio
Di Raimondo, Francesco
Palumbo, Giuseppe A.
α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title_full α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title_fullStr α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title_full_unstemmed α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title_short α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
title_sort α-lipoic acid reduces iron-induced toxicity and oxidative stress in a model of iron overload
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387298/
https://www.ncbi.nlm.nih.gov/pubmed/30708965
http://dx.doi.org/10.3390/ijms20030609
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