Real-Time Interferometric Refractive Index Change Measurement for the Direct Detection of Enzymatic Reactions and the Determination of Enzyme Kinetics

Back scatter interferometry (BSI) is a sensitive method for detecting changes in the bulk refractive index of a solution in a microfluidic system. Here we demonstrate that BSI can be used to directly detect enzymatic reactions and, for the first time, derive kinetic parameters. While many methods in...

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Detalles Bibliográficos
Autores principales: Jepsen, Søren T., Jørgensen, Thomas M., Sørensen, Henrik S., Kristensen, Søren R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387378/
https://www.ncbi.nlm.nih.gov/pubmed/30696020
http://dx.doi.org/10.3390/s19030539

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