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BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue
BACKGROUND: BAM15 is a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage. The purpose of our study was to investigate the effect of BAM15 on apoptosis during 5-day transportation of human-induced...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387563/ https://www.ncbi.nlm.nih.gov/pubmed/30795805 http://dx.doi.org/10.1186/s13287-019-1151-y |
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author | Tang, Mingjun Luo, Ziming Wu, Yihui Zhuang, Jing Li, Kaijing Hu, Dongpeng Rong, Huifeng Xian, Bikun Ge, Jian |
author_facet | Tang, Mingjun Luo, Ziming Wu, Yihui Zhuang, Jing Li, Kaijing Hu, Dongpeng Rong, Huifeng Xian, Bikun Ge, Jian |
author_sort | Tang, Mingjun |
collection | PubMed |
description | BACKGROUND: BAM15 is a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage. The purpose of our study was to investigate the effect of BAM15 on apoptosis during 5-day transportation of human-induced pluripotent stem (hiPS)-differentiated retinal tissue. METHODS: Retinal tissues of 30 days and 60 days were transported with or without BAM15 for 5 days in the laboratory or by real express. Immunofluorescence staining of apoptosis marker cleaved caspase3, proliferation marker Ki67, and neural axon marker NEFL was performed. And expression of apoptotic-related factors p53, NFkappaB, and TNF-a was detected by real-time PCR. Also, location of ganglion cells, photoreceptor cells, amacrine cells, and precursors of neuronal cell types in retinal tissue was stained by immunofluorescence after transportation. Furthermore, cell viability was assessed by CCK8 assay. RESULTS: Results showed transportation remarkably intensified expression of apoptotic factor cleaved caspase3, p53, NFkappaB, and TNF-a, which could be reduced by supplement of BAM15. In addition, neurons were severely injured after transportation, with axons manifesting disrupted and tortuous by staining NEFL. And the addition of BAM15 in transportation was able to protect neuronal structure and increase cell viability without affecting subtypes cells location of retinal tissue. CONCLUSIONS: BAM15 might be used as a protective reagent on apoptosis during transporting retinal tissues, holding great potential in research and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-019-1151-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6387563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63875632019-03-04 BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue Tang, Mingjun Luo, Ziming Wu, Yihui Zhuang, Jing Li, Kaijing Hu, Dongpeng Rong, Huifeng Xian, Bikun Ge, Jian Stem Cell Res Ther Research BACKGROUND: BAM15 is a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage. The purpose of our study was to investigate the effect of BAM15 on apoptosis during 5-day transportation of human-induced pluripotent stem (hiPS)-differentiated retinal tissue. METHODS: Retinal tissues of 30 days and 60 days were transported with or without BAM15 for 5 days in the laboratory or by real express. Immunofluorescence staining of apoptosis marker cleaved caspase3, proliferation marker Ki67, and neural axon marker NEFL was performed. And expression of apoptotic-related factors p53, NFkappaB, and TNF-a was detected by real-time PCR. Also, location of ganglion cells, photoreceptor cells, amacrine cells, and precursors of neuronal cell types in retinal tissue was stained by immunofluorescence after transportation. Furthermore, cell viability was assessed by CCK8 assay. RESULTS: Results showed transportation remarkably intensified expression of apoptotic factor cleaved caspase3, p53, NFkappaB, and TNF-a, which could be reduced by supplement of BAM15. In addition, neurons were severely injured after transportation, with axons manifesting disrupted and tortuous by staining NEFL. And the addition of BAM15 in transportation was able to protect neuronal structure and increase cell viability without affecting subtypes cells location of retinal tissue. CONCLUSIONS: BAM15 might be used as a protective reagent on apoptosis during transporting retinal tissues, holding great potential in research and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-019-1151-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-02-22 /pmc/articles/PMC6387563/ /pubmed/30795805 http://dx.doi.org/10.1186/s13287-019-1151-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Tang, Mingjun Luo, Ziming Wu, Yihui Zhuang, Jing Li, Kaijing Hu, Dongpeng Rong, Huifeng Xian, Bikun Ge, Jian BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title | BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title_full | BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title_fullStr | BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title_full_unstemmed | BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title_short | BAM15 attenuates transportation-induced apoptosis in iPS-differentiated retinal tissue |
title_sort | bam15 attenuates transportation-induced apoptosis in ips-differentiated retinal tissue |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387563/ https://www.ncbi.nlm.nih.gov/pubmed/30795805 http://dx.doi.org/10.1186/s13287-019-1151-y |
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