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Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway

OBJECTIVE: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI). MATERIALS AND METHODS: Forty Sprague Dawley rats were randomly divided into following four groups: sham group,...

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Autores principales: Chen, Xiao-jie, Ren, Shu-min, Dong, Jian-zeng, Qiu, Chun-guang, Chen, Ying-wei, Tao, Hai-long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387611/
https://www.ncbi.nlm.nih.gov/pubmed/30858695
http://dx.doi.org/10.2147/DDDT.S191537
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author Chen, Xiao-jie
Ren, Shu-min
Dong, Jian-zeng
Qiu, Chun-guang
Chen, Ying-wei
Tao, Hai-long
author_facet Chen, Xiao-jie
Ren, Shu-min
Dong, Jian-zeng
Qiu, Chun-guang
Chen, Ying-wei
Tao, Hai-long
author_sort Chen, Xiao-jie
collection PubMed
description OBJECTIVE: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI). MATERIALS AND METHODS: Forty Sprague Dawley rats were randomly divided into following four groups: sham group, I/R group and EGb 761 groups (20 and 40 mg/kg). MIRI model was established after 14 days of administration. The myocardial infarct size and myocardial histology were measured and compared. Meanwhile, the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin T (TnT), TNF-α, IL-6, IL-1β, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were evaluated. Western blot was used to detect the expression of Caspase-3, Bax, Bcl-2, HO-1, Nrf2, Akt, p-Akt and nuclear protein Nrf2. RESULTS: The levels of infarct size, CK-MB, LDH, TnT, TNF-α, IL-6 and IL-1β in the EGb 761 groups were significantly lower than those in the ischemia/reperfusion (I/R) group. The content of MDA was lower in the myocardium, whereas the activities of SOD and GSH-Px were higher than those in the I/R group. The expressions of Caspase-3 and Bax in the EGb 761 groups were significantly lower than those in the I/R group, whereas the expressions of Bcl-2, p-Akt and HO-1 and nuclear protein Nrf2 in the EGb 761 groups were higher than those in the I/R group. CONCLUSION: EGb 761 might inhibit the apoptosis of myocardial cells and protect the myocardium by activating the Akt/Nrf2 pathway, increasing the expression of HO-1, decreasing oxidative stress and repressing inflammatory reaction.
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spelling pubmed-63876112019-03-11 Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway Chen, Xiao-jie Ren, Shu-min Dong, Jian-zeng Qiu, Chun-guang Chen, Ying-wei Tao, Hai-long Drug Des Devel Ther Original Research OBJECTIVE: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI). MATERIALS AND METHODS: Forty Sprague Dawley rats were randomly divided into following four groups: sham group, I/R group and EGb 761 groups (20 and 40 mg/kg). MIRI model was established after 14 days of administration. The myocardial infarct size and myocardial histology were measured and compared. Meanwhile, the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin T (TnT), TNF-α, IL-6, IL-1β, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were evaluated. Western blot was used to detect the expression of Caspase-3, Bax, Bcl-2, HO-1, Nrf2, Akt, p-Akt and nuclear protein Nrf2. RESULTS: The levels of infarct size, CK-MB, LDH, TnT, TNF-α, IL-6 and IL-1β in the EGb 761 groups were significantly lower than those in the ischemia/reperfusion (I/R) group. The content of MDA was lower in the myocardium, whereas the activities of SOD and GSH-Px were higher than those in the I/R group. The expressions of Caspase-3 and Bax in the EGb 761 groups were significantly lower than those in the I/R group, whereas the expressions of Bcl-2, p-Akt and HO-1 and nuclear protein Nrf2 in the EGb 761 groups were higher than those in the I/R group. CONCLUSION: EGb 761 might inhibit the apoptosis of myocardial cells and protect the myocardium by activating the Akt/Nrf2 pathway, increasing the expression of HO-1, decreasing oxidative stress and repressing inflammatory reaction. Dove Medical Press 2019-02-13 /pmc/articles/PMC6387611/ /pubmed/30858695 http://dx.doi.org/10.2147/DDDT.S191537 Text en © 2019 Chen et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Chen, Xiao-jie
Ren, Shu-min
Dong, Jian-zeng
Qiu, Chun-guang
Chen, Ying-wei
Tao, Hai-long
Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title_full Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title_fullStr Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title_full_unstemmed Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title_short Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
title_sort ginkgo biloba extract-761 protects myocardium by regulating akt/nrf2 signal pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387611/
https://www.ncbi.nlm.nih.gov/pubmed/30858695
http://dx.doi.org/10.2147/DDDT.S191537
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