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The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women

Childhood maltreatment (CM) is associated with an increased risk for the development of psychiatric and somatic disorders in later life. A potential link could be oxidative stress, which is defined as the imbalance between the amount of reactive oxygen species (ROS) and the neutralizing capacity of...

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Autores principales: Boeck, Christina, Gumpp, Anja M., Koenig, Alexandra M., Radermacher, Peter, Karabatsiakis, Alexander, Kolassa, Iris-Tatjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387959/
https://www.ncbi.nlm.nih.gov/pubmed/30833908
http://dx.doi.org/10.3389/fpsyt.2019.00023
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author Boeck, Christina
Gumpp, Anja M.
Koenig, Alexandra M.
Radermacher, Peter
Karabatsiakis, Alexander
Kolassa, Iris-Tatjana
author_facet Boeck, Christina
Gumpp, Anja M.
Koenig, Alexandra M.
Radermacher, Peter
Karabatsiakis, Alexander
Kolassa, Iris-Tatjana
author_sort Boeck, Christina
collection PubMed
description Childhood maltreatment (CM) is associated with an increased risk for the development of psychiatric and somatic disorders in later life. A potential link could be oxidative stress, which is defined as the imbalance between the amount of reactive oxygen species (ROS) and the neutralizing capacity of anti-oxidative defense systems. However, the findings linking CM with oxidative stress have been inconsistent so far. In this study, we aimed to further explore this association by investigating biological markers of DNA and lipid damage due to oxidation in a comprehensive approach over two study cohorts of postpartum women (study cohort I and study cohort II). The severity of CM experiences (maltreatment load) was assessed in both studies using the Childhood Trauma Questionnaire. In study cohort I (N = 30), we investigated whether CM was associated with higher levels of structural DNA damage in peripheral blood mononuclear cells (PBMC) by two methods that are highly sensitive for detecting nuclear DNA strand breaks (comet assay and γH2AX staining). In study cohort II (N = 117), we then assessed in a larger cohort, that was specifically controlled for potential confounders for oxidative stress measurements, two established serum and plasma biomarkers of oxidative stress, one representing oxidative DNA and RNA damage (8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine; 8-OH(d)G) and the other representing lipid peroxidation (8-isoprostane). In study cohort I, the analyses revealed no significant main effects of maltreatment load on cellular measures of nuclear DNA damage. The analyses of peripheral oxidative stress biomarkers in study cohort II revealed a significant main effect of maltreatment load on free 8-isoprostane plasma levels, but not on total 8-isprostane plasma levels and 8-OH(d)G serum levels. Taken together, by combining different methods and two study cohorts, we found no indications for higher oxidative DNA damages with higher maltreatment load in postpartum women. Further research is needed to investigate whether this increase in free 8-isoprostane is a marker for oxidative stress or whether it is instead functionally involved in ROS-related signaling pathways that potentially regulate inflammatory processes following a history of CM.
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spelling pubmed-63879592019-03-04 The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women Boeck, Christina Gumpp, Anja M. Koenig, Alexandra M. Radermacher, Peter Karabatsiakis, Alexander Kolassa, Iris-Tatjana Front Psychiatry Psychiatry Childhood maltreatment (CM) is associated with an increased risk for the development of psychiatric and somatic disorders in later life. A potential link could be oxidative stress, which is defined as the imbalance between the amount of reactive oxygen species (ROS) and the neutralizing capacity of anti-oxidative defense systems. However, the findings linking CM with oxidative stress have been inconsistent so far. In this study, we aimed to further explore this association by investigating biological markers of DNA and lipid damage due to oxidation in a comprehensive approach over two study cohorts of postpartum women (study cohort I and study cohort II). The severity of CM experiences (maltreatment load) was assessed in both studies using the Childhood Trauma Questionnaire. In study cohort I (N = 30), we investigated whether CM was associated with higher levels of structural DNA damage in peripheral blood mononuclear cells (PBMC) by two methods that are highly sensitive for detecting nuclear DNA strand breaks (comet assay and γH2AX staining). In study cohort II (N = 117), we then assessed in a larger cohort, that was specifically controlled for potential confounders for oxidative stress measurements, two established serum and plasma biomarkers of oxidative stress, one representing oxidative DNA and RNA damage (8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine; 8-OH(d)G) and the other representing lipid peroxidation (8-isoprostane). In study cohort I, the analyses revealed no significant main effects of maltreatment load on cellular measures of nuclear DNA damage. The analyses of peripheral oxidative stress biomarkers in study cohort II revealed a significant main effect of maltreatment load on free 8-isoprostane plasma levels, but not on total 8-isprostane plasma levels and 8-OH(d)G serum levels. Taken together, by combining different methods and two study cohorts, we found no indications for higher oxidative DNA damages with higher maltreatment load in postpartum women. Further research is needed to investigate whether this increase in free 8-isoprostane is a marker for oxidative stress or whether it is instead functionally involved in ROS-related signaling pathways that potentially regulate inflammatory processes following a history of CM. Frontiers Media S.A. 2019-02-18 /pmc/articles/PMC6387959/ /pubmed/30833908 http://dx.doi.org/10.3389/fpsyt.2019.00023 Text en Copyright © 2019 Boeck, Gumpp, Koenig, Radermacher, Karabatsiakis and Kolassa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Psychiatry
Boeck, Christina
Gumpp, Anja M.
Koenig, Alexandra M.
Radermacher, Peter
Karabatsiakis, Alexander
Kolassa, Iris-Tatjana
The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title_full The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title_fullStr The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title_full_unstemmed The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title_short The Association of Childhood Maltreatment With Lipid Peroxidation and DNA Damage in Postpartum Women
title_sort association of childhood maltreatment with lipid peroxidation and dna damage in postpartum women
topic Psychiatry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387959/
https://www.ncbi.nlm.nih.gov/pubmed/30833908
http://dx.doi.org/10.3389/fpsyt.2019.00023
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