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Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1

The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, t...

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Autores principales: Espinosa Ruiz, Cristobal, Manuguerra, Simona, Cuesta, Alberto, Santulli, Andrea, Messina, Concetta M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388118/
https://www.ncbi.nlm.nih.gov/pubmed/30736298
http://dx.doi.org/10.3390/ijerph16030474
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author Espinosa Ruiz, Cristobal
Manuguerra, Simona
Cuesta, Alberto
Santulli, Andrea
Messina, Concetta M.
author_facet Espinosa Ruiz, Cristobal
Manuguerra, Simona
Cuesta, Alberto
Santulli, Andrea
Messina, Concetta M.
author_sort Espinosa Ruiz, Cristobal
collection PubMed
description The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, the quantification of proteins related to cell cycle and DNA repair (p53), cell proliferation (extracellular signal–regulated kinase 1 (ERK1)), energetic restriction (hypoxia-inducible factor 1 (HIF1)), and redox status (Nuclear factor erythroid 2–related factor 2 (NRF2)) was also determined after prolonged exposure (7–15 days) by immunoblotting. Our results demonstrated that rising concentrations of PBDEs exposure-induced oxidative stress, and that this event modulates different cell pathways related to cell cycle, cell signaling, and energetic balance in the long term, indicating the negative impact of sub-lethal dose exposure to cell homeostasis.
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spelling pubmed-63881182019-02-27 Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1 Espinosa Ruiz, Cristobal Manuguerra, Simona Cuesta, Alberto Santulli, Andrea Messina, Concetta M. Int J Environ Res Public Health Article The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, the quantification of proteins related to cell cycle and DNA repair (p53), cell proliferation (extracellular signal–regulated kinase 1 (ERK1)), energetic restriction (hypoxia-inducible factor 1 (HIF1)), and redox status (Nuclear factor erythroid 2–related factor 2 (NRF2)) was also determined after prolonged exposure (7–15 days) by immunoblotting. Our results demonstrated that rising concentrations of PBDEs exposure-induced oxidative stress, and that this event modulates different cell pathways related to cell cycle, cell signaling, and energetic balance in the long term, indicating the negative impact of sub-lethal dose exposure to cell homeostasis. MDPI 2019-02-06 2019-02 /pmc/articles/PMC6388118/ /pubmed/30736298 http://dx.doi.org/10.3390/ijerph16030474 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Espinosa Ruiz, Cristobal
Manuguerra, Simona
Cuesta, Alberto
Santulli, Andrea
Messina, Concetta M.
Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title_full Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title_fullStr Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title_full_unstemmed Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title_short Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1
title_sort oxidative stress, induced by sub-lethal doses of bde 209, promotes energy management and cell cycle modulation in the marine fish cell line saf-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388118/
https://www.ncbi.nlm.nih.gov/pubmed/30736298
http://dx.doi.org/10.3390/ijerph16030474
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